用于脑缺血小鼠海马SOD1表达的改进灌注固定法  

Application of Modified Perfusion Fixation in the Study on the Expression of Copper/zinc Superoxide Dismutase in Mouse Hippocampus after Transient Forebrain Ischemia

作　　者：李宝红[1] 贺旭[1] 于培兰[1] 马惠苹[1] 孙林[1] 陈瑞[1] 鄢雯[2] 

机构地区：[1]首都医科大学生化系细胞与分子生物学教学实验中心,北京100069 [2]首都医科大学燕京医学院生物化学教研室,北京101300

出　　处：《动物学杂志》2007年第4期70-74,共5页Chinese Journal of Zoology

基　　金：北京市教委基金资助项目(NoKM200510025004)

摘　　要：常规灌注固定法多用于兔和大鼠等较大动物,并存在一些不足。改进了灌注固定法流程、灌注溶液的配方、流速、用量以及灌注装置,将其用于在显微操作下制备的缺血再灌注C57BL/6N小鼠模型,并对其海马进行H.E染色和免疫组织化学SOD1基因表达。结果显示,改进的灌注固定法使组织切片结构更加清晰,海马免疫阳性神经元定位于胞浆。缺血再灌注组(24hI/R)海马神经元SOD1表达比假手术对照组(sham-o)减少,而高压氧治疗组(24hHBO)SOD1表达有所恢复。表明改进的灌注固定法用于缺血再灌注C57BL/6N小鼠海马SOD1基因表达效果良好,结果可靠。实验结果提示,高压氧的治疗机制之一可能是通过增加SOD1基因表达而实现的。Routine perfusion fixation has been used in rabbits or rats, but this method has some shortage. An improved perfusion fixation method was used in C57 BL/6N mouse ischemia-perfusion model. Samples were observed with H. E staining and immunohistochemistry staining to study the expression of copper/zinc superoxide dismutase （ SOD1 ） in hippocampus of C57 BL/6N mouse after transient forebrain ischemia. Results showed that the tissue structure become clearer when the modified perfusion fixation method was used ： masculine neurons of hippocampus were located in the cytoplasm.The SOD1 expression level of the ischemia-reperfusion （24 h I/R） group was lower than that of shamoperated （sham-o） control, but that of hyperbaric oxygen treatment （24 h HBO） group almost recovered. These results illustrated that the modified perfusion fixation was good for immunohistochemistry in C57 BL/6N mouse hippocampus after transient forebrain ischemia-reperfusion. The results also indicated that one of the mechanisms of HBO therapy may be achieved by the increase of SODlexpression level.

关 键 词：C57 BL/6N小鼠 缺血再灌注 免疫组织化学 超氧化物歧化酶-1(SOD1) 高压氧 灌注固定 

分 类 号：R-332[医药卫生]