Paenibacillus sp. MY03菌株产胞外琼胶酶的条件优化  

Optimizing Conditions for the Production of Agarase from Paenibacillus sp. Strain MY03

作　　者：古静燕[1] 韩文君[1] 李天东[1] 王君明 阮期平[1] 

机构地区：[1]绵阳师范学院分子生物学与生物制药重点实验室,四川绵阳621000 [2]绵阳市惠泽生物技术有限责任公司,四川绵阳621000

出　　处：《绵阳师范学院学报》2007年第5期55-58,共4页Journal of Mianyang Teachers＇ College

基　　金：绵阳师范学院自然科学重点项目(MA200412;2004011);绵阳市应用与开发技术资金项目(2005BS001-2);四川省教育厅青年基金项目(2005B049)

摘　　要：Paenibacillus sp.MY03菌株是从柏树根部土壤中分离的1株具有较高胞外琼胶酶活性的细菌。为深入开发该菌株的胞外琼胶酶资源,结合酶的活性测定,进行了最适产酶温度、诱导型底物的起始浓度、起始pH值和培养时间等培养条件的单因子实验和验证性实验。结果表明:在该研究条件下,添加质量体积比为0.05%的琼脂粉、培养基起始pH值7.0、37℃培养60h后,MY03菌株的胞外琼胶酶产量进入稳定状态,测得粗酶液的酶活为123.50 U/mL。Paenibacillus sp. MY03 is a bacterium with high agarase activity which has been isolated from the soil of cypress roots. In order to promote the exterior agarase development, growth conditions of MY03 strain have been optimized. As a result, 60 hours after its fostering at 37℃, with the inducer of agar powder at a coneentration of 0.05% and the starting pH of 7.0, MY03 strain shows a high stability of agar- producing, which might be helpful for deep research of agarase and the encoding of genes.

关 键 词：Paenibaillus 琼胶酶 条件优化 

分 类 号：Q93-331[生物学—微生物学]