穹窿海马伞切断与卵巢切除大鼠脑内β淀粉样肽1-40的变化  

Expression of β-amyloid1～40 in rat′s brain with fornix/hippocampal fimbria transection and ovariectomy

作　　者：辛萍[1] 郭宗君[2] 金丽英[2] 董增义[3] 

机构地区：[1]青岛大学医学院附属医院检验科,山东青岛266003 [2]青岛大学医学院附属医院脑血管病研究所,山东青岛266003 [3]青岛大学医学院附属医院儿科研究所,山东青岛2660032

出　　处：《中国老年学杂志》2007年第14期1329-1331,共3页Chinese Journal of Gerontology

基　　金：山东省科技厅科研基金(003130110;22130109)

摘　　要：目的观察穹窿海马伞切断与卵巢切除大鼠脑内不同部位β淀粉样肽1-40(Aβ1-40)表达的变化,探讨模拟实验性Alzheimer病(AD)动物模型的方法。方法成年健康雌性Wistar大鼠28只,按2×2方差分析的方法随机分为4组:穹窿海马伞切断组(FF组)、卵巢切除组(OVX组)、卵巢切除加穹窿海马伞切断组(OF组)、对照组,每组7只。穹窿海马伞切断参照Klein等的方法进行。卵巢切除参照Singh等的方法进行。对照组除不切除卵巢和不切断穹窿海马伞外,其余步骤同模型组。免疫细胞化学染色观察皮质区、海马CA1区、杏仁复合体区、基底前脑Mey-nert核等部位Aβ1-40阳性细胞表达变化。结果经F分析,双侧卵巢切除和双侧穹窿海马伞切断存在显著的主效应和交互效应(P<0.001)。双侧穹窿海马伞切断或双侧卵巢切除主效应均可导致各脑区Aβ1-40阳性细胞表达与对照组比较显著增多(P<0.01)。穹窿海马伞切断加卵巢切除二者共同作用在海马CA1区、杏仁核复合体区、Meynert核区具有显著的交互效应,表现为OF组Aβ1-40阳性细胞表达与FF组和OVX组比较显著增多(P<0.05)。结论双侧穹窿海马伞切断与卵巢切除存在显著的交互效应,表现为大鼠多个脑内Aβ1-40表达显著增多,可以作为模拟实验性AD动物模型的方法。Objective To observe expressive variety of β-amyloidl -40 （AIM -40） in different sections of the rat brain with fornix/hippocampal fimbria transection and ovariectomy to set up the animal model of Alzheimer＇s disease. Methods 28 healthy female Wistar rots were randomly divided into 4 groups according to 2 × 2 ANOVA design ： （1）fomix/hippocampal fimbria （FF） transection group ; （2）ovariectomized （OVX） group; （3）OVX and FF transaction （OF） group; （4）control group, 7 rats in every group. The expressive varieties of Aβ1 -40 immunoreaetive cell in cerebral cortex, hippocampus CA1, almond complex and Meynert nucleus were observed by immunohistochemical way. Results According to F analysis, FF and OVX groups had prominently main effect in the brain areas and reciprocation effect in hippocampus CA1, almond complex and Meynert nucleus （P 〈 0. 001 ） resulting in the increased expression of Aβ1 - 40 of FF and OVX groups than that in control group （P 〈0. 01 ） and the enhanced expression of Aβ1 -40 in OF group than that in FF and OVX groups （P 〈0. 05 ）. Conclusions FF and OVX have obvious reciprocation effect, presenting significantly increased expression of Aβ1 - 40, which could be served as the method for establishing Alzheimer＇s disease animal model.

关 键 词：穹窿下器官 海马 卵巢切除术 Β淀粉样肽 鼠 

分 类 号：R741.02[医药卫生—神经病学与精神病学]