不同粒径银粒子的体外细胞毒性比较  

Comparison of in vitro Cytotoxicity of Silver Particles with Different Sizes

作　　者：熊玲[1] 蒋学华[1] 陈亮[2] 汤京龙[2] 奚廷斐[2] 

机构地区：[1]四川大学华西药学院,成都610044 [2]中国药品生物制品检定所,北京100050

出　　处：《中国生物医学工程学报》2007年第4期600-604,共5页Chinese Journal of Biomedical Engineering

摘　　要：目的 比较纳米银粉末与微米银粉末体外细胞毒性的差异,并初步探讨毒性机制与粒径大小的相关性.方法 四种不同粒径银粒子通过扫描电镜确定其粒径分布后,制备成不同浓度的含银培养液与L929细胞接触培养,通过倒置相差显微镜观察其形态,采取MTT（四唑盐）比色法量化细胞毒性,计算相对增值率（RGR）,并进行毒性评价.结果 当银浓度在一定范围内（2.5～25）μg//mL,纳米银粒子呈现轻微细胞毒性（0～1）级,RGR与含银量呈量-效关系;随着浓度增高到大于50μg/mL时,与之共培养的细胞,形态上发生较大改变,显示明显的细胞毒性,量-效相关性也随之消失.粒径较小的微米银在银浓度达到250μg/mL时显示明显的细胞毒性.而粒径较大的两组微米银粒子在所有试验浓度下,共培养的细胞生长良好,未见明显的细胞毒性.结论 粒径不同的银粒子的体外细胞毒性有较大差异.同等剂量下,纳米级的银粒子和粒径较小的微米级银粒子,比粒径较大的微米级银粒子的体外细胞毒性更大.Object To compare the cellular toxic response with respect to the different sizes of silver particles and investigate the relationship between size and toxicity. Methods Size distribution of silver particles was confirmed by scanning electron microscopy （SEM）, L929 cells were used in this study. Silver particles with different sizes in various concentrations were contact with the cells respectively. The cellular morphology was observed by phase contrast inverted microscopy and proliferation of the cells was examined using mitochondrial function （MTT） assay. Relative growth rate （RGR） was calculated and cytotoxicity grade was evaluated. Results Silver nanoparticles showed low toxicity when silver concentrations within 2.5-25μg/mL, the cell RGR decreased as the concentration increasing. However, when silver concentration reached 50μg/mL, it showed significant toxicity and the dose-effect response disappeared at the same time. Silver microparticles of larger size showed significant toxicity when concentration reached 250μg/mL. Among all concentrations, only cells contacted with silver microparticles of larger size showed normal morphology and proliferation. Conclusions There was different cytotoxicity between nanoscale and microscale silver. It showed silver nanoparticles and smaller silver microparticles have higher cytotoxicity than lager silver microparticles at the same concentrations.

关 键 词：纳米银 微米银 细胞毒性 MTT比色法 

分 类 号：R318.08[医药卫生—生物医学工程]