Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins  

作　　者：马洪明 麦康森 刘付志国 徐玮 

机构地区：[1]Institute of Oceanology, Chinese Academy of Sciences [2]Life Science and Technology Division, Ocean University of China

出　　处：《Chinese Journal of Oceanology and Limnology》2007年第3期304-309,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China (973 Program, No. G1999012012);the Key Laboratory of Mariculture, Ministry of Education (No. 2004024)

摘　　要：An actin gene (CfACT1) was cloned by using RT-PCR, 3’and 5’RACE from hemocytes of the sea scallop Chlamys farreri. The full length of the transcript is 1 535 bp, which contains a long 3’ un-translated region of 436bp and 59bp of a 5’ un-translated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparisons indicated that CfACT1 is more closely related to vertebrate cytoplasmic actins than muscle types. Phylogenetic analysis showed that molluscan actins could be generally divided into two categories: muscle and cytoplasmic, although both are similar to vertebrate cytoplasmic actins. It was also inferred that different isotypes existed in muscle or cytoplasma in mollusks. The genomic sequence of CfACT1 was cloned and sequenced. Only one intron was detected: it was located between codons 42 and 43 and different from vertebrate actin genes.An actin gene （CfACT1） was cloned by using RT-PCR, 3＇ and 5＇RACE from hemocytes of the sea scallop Chlamys farreri. The full length of the transcript is 1 535 bp, which contains a long 3＇ un-translated region of 436bp and 59bp of a 5＇ un-translated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparisons indicated that CfACT1 is more closely related to vertebrate cytoplasmic actins than muscle types. Phylogenetic analysis showed that molluscan actins could be generally divided into two categories： muscle and cytoplasmic, although both are similar to vertebrate cytoplasmic actins. It was also inferred that different isotypes existed in muscle or cytoplasma in mollusks. The genomic sequence of CfACT1 was cloned and sequenced. Only one intron was detected： it was located between codons 42 and 43 and different from vertebrate actin genes.

关 键 词：Chlamysfarreri ACTIN CLONE phylogenetic analysis INTRON 

分 类 号：Q953[生物学—动物学] Q78