甲基泼尼松龙冲击疗法对急性视神经炎外周血IL-12和IL-18表达的影响  

Expression of IL-12 and IL-18 in patients with acute optic neuritis before and after methylprednisolone pulse therapy

作　　者：于旭辉[1] 葛红岩[1] 张蕾[1] 宋晗[1] 遇颖[1] 王宇虹[2] 

机构地区：[1]哈尔滨医科大学附属第一医院眼科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院干部一病房

出　　处：《徐州医学院学报》2007年第7期464-466,共3页Acta Academiae Medicinae Xuzhou

基　　金：黑龙江省卫生厅医学科研专题项目(2006-018);黑龙江省攻关国际合作项目(WC05C04)

摘　　要：目的探讨甲基泼尼松龙冲击疗法(methylprednisolone pulse therapy,MPPT)对急性视神经炎(acuteoptic neuritis,AON)患者外周血促炎症细胞因子白细胞介素12(IL-12)和白细胞介素18(IL-18)表达的影响。方法用酶联免疫吸附试验(ELISA)检测MPPT治疗前后AON患者外周血IL-12和IL-18的表达变化;同时用逆转录-聚合酶链反应(RT-PCR)方法检测AON患者MPPT治疗前后外周血T淋巴细胞表面IL-12受体(IL-12R)mRNA与IL-18受体(IL-18R)mRNA的表达变化。结果27例经MPPT治疗的AON患者,治疗前血清IL-12含量为(45.6±12.2)ng/L,治疗后为(17.1±4.7)ng/L,与治疗前比较显著降低(P<0.05)。治疗前血清IL-18含量为(157.5±39.3)ng/L,治疗后为(126.2±22.6)ng/L,与治疗前比较显著降低(P<0.05)。治疗前外周血T淋巴细胞IL-12R mRNA的表达比为0.2948±0.098,治疗后为0.1507±0.087,与治疗前比较显著降低(P<0.05);治疗前IL-18R mRNA的表达比为0.5352±0.1134,治疗后为0.2843±0.1127,与治疗前比显著降低(P<0.05)。结论MPPT治疗可以通过降低AON患者促炎细胞因子IL-12与IL-18的表达,发挥重要的抑炎作用。Objective To investigate the expression and relationship between IL - 12 and IL - 18 in acute optic neuritis （AON） before and after methylprednisolone pulse therapy （MPPT）. Method ELISA was employed to determine the expression of IL - 12 and IL - 18 in serum in 27 patients with AON before and after MPPT. RT - PCR analysis was employed to demonstrate the expression of IL - 12 and IL - 18 receptors in peripheral lymphocytes. Results The serum level of IL - 12 was decreased from （45.6 ± 12.2 ） ng/L to （ 17.1 ± 4.7 ） ng/L after MPPT （ P 〈 0.01 ） ; that of IL - 18 , from （ 157.5 ± 39.3 ） ng/L to （ 126.2 ± 22.6） ng/L （ P 〈 0.05 ）. The expression of IL12R mRNA was decreased after MPPT too （0.2948 vs. 0. 1507, P 〈0.05） ; and sowas that of IL18R mRNA （0.5352 vs. 0.2843, P 〈 0.05 ）. The expressions of IL - 12 and IL - 18 as well as that of their receptors were positively correlated. Conclusion Mythylprednisolone can exert its important antiphlogistic action against AON by inhibiting the expression of the proinflam- matory cytokines IL- 2 and IL- 18.

关 键 词：急性视神经炎 甲基泼尼松龙 冲击疗法 白细胞介素12 白细胞介素18 

分 类 号：R774.610[医药卫生—眼科]