血红素加氧酶-1基因在三氧化二砷诱导食管癌EC9706细胞凋亡过程中的表达  

The expression of HO-1gene in the apoptosis process of EC9706 cell induced by As_2O_3

作　　者：马会敏[1] 金国平[1] 王献伟[1] 单杰[1] 

机构地区：[1]郑州大学基础医学院生物化学与分子生物学教研室,河南郑州450001

出　　处：《河南医学研究》2007年第2期118-120,共3页Henan Medical Research

基　　金：河南省科委资助项目(054022700);郑州市科委资助项目(05SGYS33211)

摘　　要：目的:检测三氧化二砷(As2O3)诱导食管癌EC9706细胞凋亡过程中血红素加氧酶-1(HO-1)基因的表达,探讨HO-1的表达与化疗药物诱导肿瘤细胞凋亡的内在联系。方法:以食管癌EC9706细胞系作为研究模型,3μmol/L AS2O3作用于EC9706细胞,镜下观察细胞形态变化;MTT法绘制细胞生长曲线;以RT-PCR方法检测HO-1mRNA的表达。结果:As2O3作用于EC9706细胞4 h即可检测到HO-1基因表达升高,12 h表达水平升至最高,24 h回落到正常水平。结论:As203诱导EC9706细胞凋亡过程中,HO-1基因表达升高,呈时间依赖性变化,可能起到短暂的对抗氧化应激作用。Objective： To detect the expression of HO-1 gene in the apoptotic process of EC9706 cell induced by As2O3,and to approach the relationship between the expression of HO-1 gene and the tumor cell apoptosis induced by chemotherapeutics. Methods ： EC9706 cell were treated with 3μmol/L As2O3 ,then to observe the change of cell＇ s appearance under microscope and record the cell growth curve using MTT method as well as detecting the expression of HO-1 mRNA with RTPCR method. Results： 4 h after cells were treated with As2O3 ,the expression of HO-1 mRNA had begin to rise . It reached the highest level at 12 h,and at 24 h,it had return to the normal level. Conclusion： In the apoptotic process of EC9706 cell induced by As2O3, the expression of HO-1 gene rises along with the time . The change may contribute to protecting tumor cells from oxidative stress temporarily.

关 键 词：食管癌EC9706细胞 血红素加氧酶-1 基因表达 三氧化二砷诱导凋亡 

分 类 号：R735.1[医药卫生—肿瘤]