胃癌c-myc c-Ha-ras基因DNA甲基化与病理的关系  

Relationship Between c-myc, c-Ha-ras Oncogenes Methylation and Pathologic Changes in Gastric Carcinoma

作　　者：房静远[1,2] 朱舜时[1,2] 施尧[1,2] 萧树东[1,2] 江绍基[1,2] 周筱梅[3] 

机构地区：[1]上海第二医科大学附属仁济医院 [2]上海市消化疾病研究所,200001 [3]上海市肿瘤研究所,200032

出　　处：《上海第二医科大学学报》1997年第1期13-15,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金!39370332

摘　　要：以限制性内切酶HpaⅡ／MspⅠ消化、Southernblot方法，分析22例进展期胃癌手术标本的癌灶、癌旁和外周正常组织细胞内c－myc、c－Ha－ras癌基因位点的DNA甲基化状态，并与其组织病理学变化相比较。结果发现1例正常组织、13例癌旁和10例癌灶细胞c－myc基因和自显影较清楚的10组标本中的4例癌旁、5例癌灶细胞内c－Ha－ras基因呈低甲基化状态。以上改变与肿瘤大体形态、组织类型、癌细胞分化程度、淋巴结转移及癌细胞浸润深度等病理学改变无明显相关性。In order to study the relationship between the DNA methylation of specific genes and pathologic changes in gastric carcinoma, we analysed the methylation status of c-myc, c - Ha- ras oncogenes by Southern blot hybridization. Genomic DNA from cancerous, paracancerous and normal areas of surgical resected samples in 22 cases of human advanced gastric carcinoma were digested with the restriction endonucleases MspⅠ/HpaⅡ which can cleavaged between methylatied and notunethylatied cytosine in their nucleotide recognition site DNA 5' - CCGG, and hybridized with cmyc, c- Ha-ras oncogene probes. Moreover the corresponding pathologic changes were observed.The resultS showed that c - myc, c - Ha - ras oncogene from cancer(10/22, 5/10) and paracancer (13/22,4/10) are hypomethylated and these are no correlation to histopathologic changes.

关 键 词：胃癌 DNA 甲基化 癌基因 病理学 

分 类 号：R735.202[医药卫生—肿瘤]