人工伴侣条件下谷胱甘肽浓度对溶菌酶复性的影响  

Effect of Glutathion Concentration on Lysozyme Refolding Assisted by Artificial Chaperones

作　　者：贾长虹[1] 卢育红[2] 

机构地区：[1]天津大学化工学院生物工程系,天津300072 [2]河北理工大学化工与生物技术学院生物技术系,河北唐山063009

出　　处：《青岛大学学报（工程技术版）》2007年第2期78-83,共6页Journal of Qingdao University(Engineering & Technology Edition)

基　　金：河北省教育厅资助项目(2004107);唐山市科技局资助项目(04204501C-20)

摘　　要：利用盐酸胍变性、二硫苏糖醇还原的溶菌酶为模型蛋白,考察人工伴侣一步法辅助溶菌酶复性时的最佳谷胱甘肽浓度。结果表明,当复性体系中溶菌酶浓度不同时,得到最大复性收率的GSSG与GSH的浓度有所差异。酶的质量浓度在0.06,0.1,0.2 mg/mL的条件下,随酶带入的还原型DTT的作用可以不予考虑,GSSG与GSH物质的量浓度比维持在1∶1时可以得到最大复性收率;酶的质量浓度在0.6 mg/mL的条件下,应当考虑DTT的作用,此时,需将变化后的GSSG与GSH物质的量浓度比维持在1∶1时,才能得到较大复性收率。In order to explore the best concentration of glutathione in the test assisting with artificial chaperones by one-step method, the hydrochloric acid-Denatured and DTT-Reduced Lysozyme are taken as the model protein. The result indicates that the ratio of GSSG and GSH varies because of different concentrations of lysozyme in refolding buffer. Without considering the effect of reduced DTT, the best refolding yield could be obtained when the concentrations of lysozyme are 0.06, 0.1 and 0.2 mg/mL and the GSSG and GSH remains 1：1. When the lysozyme concentration is 0. 6 mg/mL, the effect of reduced DTT should be considered, the better refolding yield can be obtained while the transformed GSSG and GSH remains 1 ： 1.

关 键 词：蛋白质复性 溶菌酶 人工伴侣 谷胱甘肽 

分 类 号：Q55-3[生物学—生物化学]