高效液相色谱法(HPLC)测定山楂片中的熊果酸和齐墩果酸  

THE DETERMINAION OF URSOLIC ACID AND OLEANOLIC ACID IN HAW PIECE BY HPLC

作　　者：袁光耀[1] 郭振福[1] 贾艳霞[1] 

机构地区：[1]河北北方学院基础部,河北张家口075131

出　　处：《食品研究与开发》2007年第8期137-139,共3页Food Research and Development

摘　　要：建立了高效液相色谱同时测定山楂片中熊果酸和齐墩果酸含量的方法。色谱条件:色谱柱Hypersil BDS C18(4.6mm×200mm,5μm),柱温为25℃,UV检测波长2l0nm,流动相为甲醇-磷酸水溶液(pH=3.0)(85∶15),流速为0.5mL/min。结果表明:熊果酸和齐墩果酸的线性范围38.0μg/mL～600μg/mL(r=0.9991)和12.5μg/mL～200μg/mL(r=0.9994),平均回收率分别为99.06%和99.17%,RSD分别为1.43%(n=5)和2.49%(n=5);该方法简便、准确,适合于山楂片中熊果酸和齐墩果酸含量的测定。A method for the determination of ursolic acid and oleanolic acid in haw piece by high performance liquid chromatography has been established. The chromatography conditions as follow： Hypersil BDS C18conlum （4.6 mm×200 mm, 5 μm）, UV detection wavelengts 210 nm. The best mobile phase was methol-water（adjusted to pH = 3.0 with H3PO4 ） （85：15） at a flow rate of 0. 5 mL/min, The conlum temperature was 25 ℃.The result showed that for Ursolic acid and Oleanolic acid the calibration curve was linear in the range of 38.0 μg/mL 600 μg/mL（r=0.999 1 ） and 12.5μg/mL-200μg/mL（ r=0.999 4）, respectively. The average recovery percent were 99.06 % and 99.17 %, the RSD were 1.43 %（n=5） and 2.49 %（n=5）, respectively .The methods is suitable for contents analysis of Ursolic acid and Oleanolic acid in haw piece.

关 键 词：高效液相色谱法 山楂片 熊果酸 齐墩果酸 测定 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]