突变型p53蛋白在二乙基亚硝胺诱发大鼠肝癌前病变中的表达  

The expression of mutant p53 (mp53) protein in rat hepatic preneoplastic lesions induced by diethylnitrosamine (DEN)

作　　者：朱焕章[1] 张新立[1] 陈意生[1] 

机构地区：[1]第三军医大学病理教研室,重庆630038

出　　处：《中华肿瘤杂志》1997年第1期22-24,共3页Chinese Journal of Oncology

基　　金：国家自然科学基金

摘　　要：目的　探讨 p53基因与肝细胞癌变的关系。方法　用免疫组化方法 ,观察二乙基亚硝胺( DEN)诱发大鼠肝癌前病变组织及对照组肝组织中突变型 p53( mp53)蛋白、胎盘型谷胱甘肽 S转移酶 ( GST- P)的表达及其与细胞增殖状态的关系。结果　实验组第 5周时仅在 2 / 5例癌前病变肝组织中检测到 mp53蛋白的表达 ,此蛋白仅存在于部分较大的肝细胞增生结节 ( HN)或 GST- P阳性灶内 ;mp53蛋白阳性的 HN或 GST- P阳性灶 ,其细胞的溴化脱氧尿嘧啶标记指数 ( Brd U- LI)较阴性者高( P<0 .0 5)。结论　 p53基因的突变可发生于肝细胞癌变的早期阶段 ,与肝细胞的异常增殖和癌变有关 ,并有可能成为新的肝癌前肿瘤基因标记物。Objective To study p53 genes related to hepatocarcinogenesis. Methods Using immunohistochemistry techniques, the relation between expression of mp53 protein, placental glutathione S transferase (GST P) and the cell proliferative activity was investigated in rat hepatic preneoplastic lesions induced by DEN and the control liver. Results Two of five rats with hepatic prenoplastic lesions had mp53 protein expression which was seen only in a portion of large sized hyperplastic nodules (HN) or GST P positive foci at 5th week. The bromodeoxyuridine labeling indeX (BrdU LI) in p53 positive HN or GST P foci was significantly higher than that of p53 negative HN or GST P negative foci respectively ( P <0.05). Conclusions Mutation of p53 gene occurs in the early stage of hepatocarcinogenesis, and is responsible for the hepatocellular proliferations and hepatocarcinogenesis, and might become an additional tumor marker.

关 键 词：肝肿瘤 二乙基亚硝胺 P53 

分 类 号：R735.702[医药卫生—肿瘤]