c-erbB-2基因对小鼠成纤维细胞生长特性的影响  

Influence of c-erbB-2 gene on growth properties of mouse fibroblast

作　　者：郝新保[1] 张盈华[1] 张利朝[1] 韩秀珍[1] 殷缨[1] 陶秦渝[1] 郝晓柯[1] 邱福海[2] 

机构地区：[1]第四军医大学唐都医院中心实验室 [2]第四军医大学唐都医院普通外科,西安710038

出　　处：《第四军医大学学报》1997年第1期23-25,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目No.39370777

摘　　要：目的:为探讨c-erbB-2基因对细胞增殖的影响.方法;本实验通过构建c-erbB-2基因的表达载体来转染NIH3T3细胞,观察了转染细胞的生长特性.结果:发现其对数生长期提前、群体倍增时间缩短(39hP<O.05),并且其克隆形成率(26%,P<0.05)、软琼脂集落形成能力(17clones/cell,P<0.05)及裸鼠致瘤能力(3/5)均明显高于对照组.结论:表明c-erbB-2基因具有一定的恶性转化能力.Objective: In order to investigate the influences of c-erbB-2 gene on cell biological properties. Methods: The c-erbB-2 gene expressing vector was constructed and transfected into the NIH3T3 cell line, and then growth properties of the transfected cell line erbBTr were observed. Results: The cell lines achieved the following properties: 1. earlier logarithmic growth phase and shorter time-span of multiplication (39 h, P<0. 05). 2. higher clone forming rate in culture media (26% , P<0. 05) and in soft arga (17 clones/cell, P <0. 05). 3. stronger ability of induced tumors in nude mice (3/5). Conclusion; It can be deduced that the c-erbB-2 gene has some neoplastic transforming ability.

关 键 词：C-ERBB-2 癌基因 成纤维细胞 乳腺肿瘤 

分 类 号：R737.310.2[医药卫生—肿瘤]