叶酸缺乏对孕鼠子代心脏发育中糖原合成酶激酶3β表达影响  

Effects of Folate Deficiency during Pregnancy on Expression of Glycogen Synthase Kinase 3β in Heart of Offspring

作　　者：张锦文[1] 韩树萍[2] 郭锡镕[3] 顾筱琪[2] 彭宇竹[4] 刘茹[3] 李静[3] 

机构地区：[1]南京医科大学附属南京妇幼保健院妇女保健科,南京210004 [2]南京医科大学附属南京妇幼保健院新生儿科,南京210004 [3]南京医科大学附属南京妇幼保健院儿童保健科,南京210004 [4]南京医科大学附属南京妇幼保健院心血管内科,南京210004

出　　处：《实用儿科临床杂志》2007年第13期969-970,共2页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(30672245);南京医科大学科技发展基金重点项目资助(2005NYDZD19);南京市卫生局医学重点科技发展项目资助(ZKX05011)

摘　　要：目的探讨孕鼠叶酸缺乏(FD)对子代心脏发育过程中GSK3β基因表达的影响。方法成熟雌性SD大鼠36只随机分为实验和对照组各18只,分别喂以缺乏叶酸和添加叶酸纯合饲料。2周后与成熟SD雄性大鼠交配,分别取孕13.5、17.5d胚胎鼠及新生鼠心脏。用逆转录聚合酶链反应(RT-PCR)检测GSK3β基因mRNA表达。结果GSK3βmRNA在孕13.5、17.5d胚胎心脏及新生鼠心脏中的表达量,实验组均显著低于对照组(Pa<0.05)。结论叶酸缺乏可影响GSK3β基因表达水平,可能导致心脏发生发育中形态改变,造成心脏功能缺陷。Objective To study the expressions of glycogen synthase kinase 3β（GSK3β） genes on the heart of offspring during the development of embryo, whose mother was deficient of folic acid. Methods Thirty - six adult female SD rats were chosen and alloratad randondy into control group （18 rats） and study group （18 rats）. The control group received nonfolic acid fodder and the study group received folic acid fodder for 2 weeks, Then the female rats in 2 groups copulated with normal male rats, The hearts of the 13.5 days,17, 5 days embryos and the newborns were obtained, The expressions of GSK3βgenes mRNA at the 3 periods were measured by reverse transcriptase - polymerase chain reaction（RBT - PCR）. Results The expression levels of GSK315 mRNA in study group were significantly weaker than that in control group in hearts of the 13, S days, 17, 5 days embryos and the newborns（ P〈0.05 ）. Conclusion Changes of expression of GSK3β mRNA may be resulted from folic acid deficiency during the pregnancy, which may lead to the malformation of the heart.

关 键 词：叶酸 心脏 胚胎 鼠 新生 糖原合成酶激酶3Β 

分 类 号：R363[医药卫生—病理学]