克劳茨基棉组织培养胚胎发生与器官分化的扫描电镜观察  

Scanning Electron Microscope Observation on Embryogenesis and Organogenesis in Tissue Culture of Gossypium klotzschanium Anderss

作　　者：张宝红[1] 丰嵘[1] 杜雄明[1] 李凤莲[1] 王春英[1] 冯新爱[1] 李秀兰[1] 

机构地区：[1]中国农业科学院棉花研究所

出　　处：《电子显微学报》1997年第2期81-86,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金

摘　　要：用扫描电镜观察克劳茨基棉不同类型的愈伤组织和胚状体。结果表明克劳茨基棉花药诱导产生的愈伤组织可分为胚性愈伤组织和非胚性愈伤组织两类；克劳茨基棉组织培养植株再生存在着两条途径，即胚胎发生途径和器官分化途径；胚状体表面光滑，不定芽表面密生着大量的表皮毛。本文提出了在扫描电镜下鉴别胚状体、不定芽。The various kinds of callus and embryoids from Gossypium klotzschanium Anderss anther have been observed under scanning electron microscope.The results indicated that the callus could be classified into two kinds of embryogenic and nonembryogenic callus,the plantlets regenerated through two pathways of embryogenesis and organogenesis.The obvious distinguish existed between embryoids and adventitious buds,embryoid with smooth surface,but adventitious bud was covered by many epidermal hairs.The morphological basis of distinguish among embryoids,adventitious buds and roots under SEM was put forward in this paper.

关 键 词：克劳茨基棉 组织培养 胚胎发生 器官分化 棉花 

分 类 号：S562.035.3[农业科学—作物学]