RECK，MMP-9及TGF-β1在胃癌组织中的表达及其相互关系  

Expression of reversion-inducing-cysteine-rich protein with Kazal motifs,matrix metalloproteinase-9 and transforming growth factor-β1 and their correlations in gastric carcinomas

作　　者：宋国庆[1] 王强[1] 

机构地区：[1]中国医科大学附属盛京医院胃肠外科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2007年第15期1731-1737,共7页World Chinese Journal of Digestology

摘　　要：目的:研究RECK,MMP-9和TGF-β1在胃癌及正常胃组织中的表达,探讨其在胃癌的发生、发展、浸润和转移中的作用.方法:选择临床及病理资料齐全的存档胃腺癌蜡块标本54例,另取正常胃黏膜标本15例作对照.采用第二代通用型二步法监测系统(PV-9000)免疫组化方法检测RECK,MMP-9及TGF-β1在胃癌及正常胃组织中的表达.结果:RECK在胃癌组织中低表达(51.9%),并随肿瘤浸润深度的加深、淋巴转移的产生、远隔转移的发生、临床分期的提高、肿瘤病理分化程度的降低而降低(P<0.05).MMP-9在胃癌组织中高表达(75.9%),并随肿瘤浸润深度的加深、淋巴转移的产生、临床分期的提高、肿瘤病理分化程度的降低而增高(P<0.05).TGF-β1在胃癌组织中高表达(77.8%),并随淋巴转移的产生、临床分期的提高、肿瘤病理分化程度的降低而增高(P<0.05).胃癌组织中RECK与MMP-9、TGF-β1的表达呈负相关(r=-0.618,P<0.001;r=-0.620,P<0.001),MMP-9与TGF-β1的表达呈正相关(r=0.716,P<0.001).结论:胃癌组织中RECK低表达,MMP-9,TGF-β1高表达.RECK,MMP-9及TGF-β1可以作为评估胃癌发生浸润转移的重要指标.AIM： To study the expression of RECK （reversioninducing-cysteine-rich protein with Kazal motifs）, MMP-9 （matrix metalloproteinase-9）, and TGF-β1 （transforming growth factor-β1） as well as their correlations in gastric cancer, and to investigate their roles and development. in the carcinogenesis and development. METHODS： PV-9000 immunohistochemistry was used to measure the expression of RECK, MMP-9, and TGF-β1 in 54 samples of gastric carcinoma selected from paraffin wax-embodied specimens with complete clinicopathological data, and another 15 cases of normal gastric mucosa. RESULTS： The expression rate of RECK in gastric cancer was low （51.9%）, and reduced with the increased invasive decreased histological depth and clinical stages, differentiation and occurrence of lymphatic and long-distance metastasis （P 〈 0.05）. The expression rate of MMP-9 in gastric cancer was high （75.9%）, and elevated with the increased invasive depth and clinical stages, decreased histological differentiation, and occurrence of lymphatic metastasis （P 〈 0.05）. The expression rate of TGF-β1 in gastric cancer was also high （77.8%）, and enhanced with the increased clinical stages, reduced histological differentiation and occurrence of lymphatic metastasis （P 〈 0.05）. The expression of RECK was negatively correlated with the expression of MMP-9 and TGF-β1 （r = -0.618, P 〈 0.001; r = -0.620, P 〈 0.001）, while the expression of MMP-9 was positively correlated with that of TGF-131 （r = 0.716, P 〈 0.001）. CONCLUSION： RECK is lowly expressed in gastric caner, but MMP-9 and TGF-β1 are highly expressed. RECK, MMP-9 and TGF-β1 may serve as the markers in the estimation of metastasis and dissemination of gatric cnacer.

关 键 词：胃癌 RECKS蛋白 基质金属蛋白酶-9 转化生长因子-Β1 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]