白藜芦醇对小鼠Lewis肺癌生长的影响及机制研究  

Influence of resveratrol on the growth of Lewis lung carcinomas in mice and its underlying mechanism

作　　者：符慧群[1] 曹培国[1] 罗红[2] 张隽[1] 

机构地区：[1]中南大学湘雅三医院肿瘤科,长沙410013 [2]中南大学湘雅二医院呼吸内科,长沙410011

出　　处：《肿瘤》2007年第7期531-534,共4页Tumor

摘　　要：目的:探讨白藜芦醇(resveratrol,Res)对小鼠Lewis肺癌生长的影响及其作用机制。方法:建立C57BL小鼠Lewis肺癌模型,将40只接种Lewis肺癌的C57BL小鼠随机分成4组:对照组、Res低剂量组(2.5mg·kg-1·d-1)、Res中剂量组(5mg·kg-1·d-1)和Res高剂量组(10mg·kg-1·d-1),每组10只。连续灌胃20d,于接种后第22d处死小鼠,检测肿瘤体积及重量,通过免疫组化法检测肿瘤组织微血管密度(MVD),采用免疫组化和RT-PCR分析肿瘤细胞血管内皮生长因子(VEGF)的表达水平,并用原位末端标记法(TUNEL)检测肿瘤细胞凋亡指数(AI)。结果:Res中、高剂量组肿瘤的生长明显受到抑制,瘤重和肿瘤体积明显低于对照组(P<0.01);其抑瘤率分别为39.04%、49.66%,明显高于Res低剂量组(12.33%),差异有显著性(P<0.01)。Res中、高剂量组VEGF表达水平及MVD明显降低,AI则明显升高,与对照组比较,差异均有显著性(P<0.01),但低剂量Res对VEGF表达、MVD及AI无明显影响(P>0.05)。结论:白藜芦醇可明显抑制小鼠Lewis肺癌的生长,其机制可能与抑制VEGF表达、降低微血管密度和促进细胞凋亡有关。Objective： To explore the influence of resveratrol （Res） on the growth of Lewis lung carcinomas in mice and the corresponding mechanism. Methods：The Lewis lung carcinoma model was established in C57BL mice. Forty C57BL mice were randomly divided into 4 groups （n = 10） ： control group, low-dose Res group （2.5 mg·kg^-1·d^-1 ）, middle-dose Res group （5 mg·kg^-1·d^-1 ） and high-dose Res group （ 10 mg·kg^-1·d^-1 ）. Mice were given intragastrically with Res for 20 d. All mice were sacrificed on d 22 after inoculation. The volume and weight of tumors were recorded. Microvessel density （MVD） in tumor tissues was measured by immunohistochemistry. The expression level of vascular endothelial growth factor （VEGF） was examined by immunohistochemistry and RT-PCR. The apoptotic index （AI） was determined by TUNEL assay. Results ： The tumor growth was suppressed significantly in Res 5 mg and 10 mg·kg^-1·d^-1 groups. The weights and volumes of Lewis lung carcinoma in both groups above were markedly decreased compared with control group （P 〈 0. 01 ）. The inhibitory rates were 39.04% and 49.66% , respectively, which was significantly higher than that in Res 2.5 mg·kg^-1·d^-1 group （ 12.33% ,P 〈0. 01 ）. Mice in Res 5 mg and 10 mg·kg^-1·d^-1 group had markedly lower levels of VEGF expression and MVD and significantly higher AI compared with control group （ P 〈 0. 01 ）. However, Res 2.5 mg·kg^-1·d^-1 had no obvious influence on VEGF expression, MVD and AI （P 〉 0. 05 ）. Conclusion： Res remarkably inhibites the growth of Lewis lung carcinoma in mice by inhibiting expression of VEGF, reducing MVD, and promoting apoptosis.

关 键 词：白藜芦醇 肺肿瘤 内皮 血管 内皮生长因子 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤] R73-361[医药卫生—临床医学]