脂肪酸合成酶及其mRNA在肺癌中的表达及临床意义  

Expression of fatty acid synthase in lung cancer and its clinical significance

作　　者：阮征[1] 花天放[2] 彭寿行[1] 薛月华[3] 胡宏慧[4] 

机构地区：[1]上海交通大学附属第一人民医院胸外科,上海200080 [2]上海交通大学附属第一人民医院普外科,上海200080 [3]上海交通大学附属第一人民医院中心实验室,上海200080 [4]上海交通大学附属第一人民医院病理科,上海200080

出　　处：《肿瘤》2007年第7期557-561,共5页Tumor

摘　　要：目的:本研究同期观察脂肪酸合成酶(fatty acid synthase,FAS)及其 mRNA在肺癌中的表达及其临床意义。方法:采用免疫组织化学EnVisionTM法和逆转录聚合酶链反应技术(RT-PCR)对67例肺癌组织进行FAS及其 mRNA的检测和分析,并与癌旁组织,正常肺组织进行对照研究。结果:FAS蛋白表达在肺癌组织中阳性率为41.8%(28/67),显著高于癌旁组织19.5%(8/41)和正常肺组织6.25%(2/32)(P<0.01)。FAS mRNA在肺癌组织中表达阳性率为67.2%(45/67),显著高于癌旁组织34.1%(14/41)和正常肺组织25.0%(8/32)(P<0.01)。半定量法测定发现FAS mRNA在阳性表达的肺癌组织中为0.975±0.326,显著高于癌旁组织0.475±0.167和正常组织0.261±0.050(P<0.01)。FAS蛋白和 mRNA的表达与患者性别,年龄,肿瘤部位,病理类型,分化程度无关(P>0.05);与肿瘤分期,淋巴结转移(P<0.01)有相关性。结论:FAS蛋白和 mRNA在肺癌组织中表达增强,提示在肿瘤的形成与演变的过程中可能存在着同步的脂肪酸代谢改变;FAS可能作为肺癌诊断的指标,同时也可为肺癌的治疗提供新的靶位。Objective ：Much evidences demonstrate that the fatty acid synthase （FAS） plays an important role in the intiation and progression of various cancers and signs of poor prognosis. But it is not clear whether there is relationship between FAS protein and mRNA expression and lung cancer. The aim of this study was to explore the expressions of FAS protein and mRNA in lung cancer and to analyze its association with clinicopathological features. Methods： Immunohistochemical EnVision^TM staining and reverse transcription polymerase chain reaction （RT-PCR） were performed to determine the protein and mRNA expressions of FAS in 67 cases with lung cancer and compared with para-cancerous and normal tissues. Results：The expressions of FAS protein was located in cell cytoplasm. The immunohistochemical EnVisionTM staining showed that the positive rate of FAS protein was 41.8% （28/67）in lung cancer tissue, which was significantly higher than para-cancerous tissue （19.5% ,8/41 ） and normal tissue （6.25% ,2/32 ） （P 〈： 0.01 ）. The expressions of FAS mRNA was positive in 67.2% （ 45/67 ） of lung cancer tissue, which was also significantly higher than para-cancerous tissue （34.1%, 14/41 ） and normal tissue（ 25.0% ,8/32 ） （P 〈 0.01 ）. The relative level of FAS mRNA in lung cancer tissues was significantly higher than para-cancerous tissue and normal tissue （0.957±0. 326 vs 0.475±0. 167 and 0.261±0.050, P 〈0.01）. The expressions of FAS protein and mRNA significantly correlated with the stage and lymph node metastasis （ P 〈 0.01 ）, but had no significant correlation with age, sex, pathological type, and cell differentiation degree. Conclusion： FAS protein and mRNA are over expressed and hyperactivated in lung cancer indicating abnormal fatty acid metabolism during the initiation and development of cancer. FAS can be considered as a valuable marker for diagnosis of lung cancer or serves as a new target for lung cancer therapy.

关 键 词：肺肿瘤 脂肪酸合成酶复合物 免疫组织化学法 逆转录聚合酶链反应 诊断 

分 类 号：R734-2[医药卫生—肿瘤] R730-2[医药卫生—临床医学]