恶性肿瘤细胞及组织中凋亡抑制蛋白livin的表达及其临床意义  

Expression of a novel inhibitor of apoptosis protein livin in malignant tumor cells and tissues and its clinical significance

作　　者：邹爱民[1] 沈建军[1] 高萍[1] 林芳[1] 张惠中[1] 

机构地区：[1]第四军医大学唐都医院中心实验室,西安710038

出　　处：《肿瘤》2007年第7期570-572,共3页Tumor

摘　　要：目的:探讨凋亡抑制蛋白livin的两种异构体livinα、和livinβ在多种肿瘤细胞系及肿瘤组织中的表达及其与各类肿瘤临床病理的关系。方法:采用RT-PCR法检测HeLa、SBC-2等12种肿瘤细胞和50例不同肿瘤组织及20例癌旁组织中livinα和livinβ的mRNA的表达水平。结果:12个细胞系中8个细胞系的livin mRNA表达为阳性,分别为HeLa、SPCA-1、SBC-2、PC14、MKN45、LOVO、HHCC、A549,其中A549为弱表达,且livinα的表达强于livinβ;HL-7702、Hep-2、7721、K562四种细胞中livin表达为阴性;50例肿瘤组织中livin mRNA表达阳性率为64.0%,而在癌旁组织中为2%。Livin mRNA的表达与肿瘤患者的肿瘤分化程度、淋巴结转移及TNM分期均无明显相关性。结论:Livin在肿瘤细胞系及肿瘤组织中表达的研究,为进一步研究livin与肿瘤的发生、发展的关系,及其以livin为靶位的肿瘤基因治疗研究提供了有力依据。Objective ： To examine and analyze the expression of two isoforms of livin （ livin α/livin β ）, a novel inhibitor of apoptosis protein （IAP） family member, in tumor cell lines and malignant tumor tissues and analyze the relationship between livin expression and clinical pathological features of different tumors. Methods： Livin mRNA expression was detected by reverse transcription polymerase chain reaction （RT-PCR） in 12 malignant tumor cell lines, 50 different malignant tumor tissues, and 20 cases of normal tissues. Results：Livin mRNA was highly expressed in seven cell lines （Hela, SPCA-1, SBC-2, PC14, MKN45, LOVO, and HHCC） and poor expressed in A549 cells. It had negative expression in other four kinds of cell lines （ HL-7702, Hep-2, 7721 and K562）. Livin mRNA was positively expressed in 32 of 50 malignant tumor cases （64.0%）. The expression of livin mRNA was at low level in paracancerous tumors tissues （2%）. Livin expression had no significant association with differentiation degree, lymph node metastasis and TNM staging of tumor patients. Conclusion：Livin expression in tumor cell lines and malignant tumor tissues provides stronger evidence for further investigating the relationship between livin and tumor initiation and development. It may serve as a new target for malignant tumor diagnosis and therapy.

关 键 词：肿瘤 实验性 凋亡抑制蛋白类 LIVIN 基因表达 

分 类 号：R73-36[医药卫生—肿瘤]