CHFR和mp53基因编码蛋白在胃癌组织中的表达及临床病理学意义  

Protein expression of checkpoint with fork head associated and ring finger and mutant p53 and their clinicopathological significances in gastric cancer

作　　者：高余佳[1] 辛彦[2] 张赛楠[2] 张家华[2] 吴东瑛[2] 

机构地区：[1]辽宁省肿瘤医院内六科,辽宁省沈阳市110042 [2]中国医科大学附属一院肿瘤研究所第四研究室,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2007年第14期1622-1627,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.30371607;教育部博士点专项基金;No.20040159021~~

摘　　要：目的:观察CHFR和P53蛋白的表达与胃癌临床病理学特征的关系,探讨其在胃癌发生发展过程中的作用及相关的分子机制.方法:利用组织芯片制作仪(美国),构建成5个包括151例胃癌及101例与其配对的正常胃黏膜、肠化生或不典型增生的组织芯片蜡块.采用Envision免疫组化二步法检测151例胃癌及101例配对癌旁胃黏膜组织中CHFR蛋白和P53蛋白的表达.结果:CHFR蛋白在非癌胃黏膜组织中阳性表达率为85.25%(52/61),在胃癌组织中阳性表达率显著降低(49.67%,75/151,P<0.05);CHFR表达下调或缺失与胃癌患者的性别显著相关,女性患者胃癌组织中CHFR表达缺失率显著高于男性患者(64% vs 43.56%,P<0.05).BorrmanⅢ+Ⅳ型胃癌组织中CHFR表达缺失率显著高于BorrmanⅠ+Ⅱ型胃癌(57.14% vs 34.78%,P<0.05).虽然不同组织学类型胃癌之间CHFR的表达无统计学差异,但本研究发现胃印戒细胞癌组CHFR表达缺失率最高(71.43%).胃癌组织中CHFR蛋白的表达缺失与肿瘤浸润深度、淋巴结转移以及mP53蛋白表达未见显著相关性(P>0.05).结论:有丝分裂前期检查点CHFR基因表达下调或缺失在胃癌中是频发事件.可能参与胃癌的发生,其与女性、弥漫浸润型胃癌发生发展的关系可能更为密切.AIM： To investigate the relationship between the expression of checkpoint with fork head associated and ring finger （CHFR） and P53 protein in gastric cancer （GC） and the clinicopathologic characteristics, and to explore the correlated molecular mechanism of CHFR and p53 genes in gastric carcinogenesis.METHODS： Five paraffin blocks of tissue mi-croarray were constructed using a Tissue Array Machine （Steve Leighton Beecher Instruments, USA）, including 151 cases of primary GC （101 cases with matched normal mucosa, intestinal metaplasia or dysplasia）. Envision immunohistochemical method was employed to detect the protein expression of CHFR and mutant p53 in GC and precancerous tissues mentioned above. RESULTS： The positive rate of CHFR protein expression in GC （49.67%, 75/151） was significantly lower than that in normal gastric mucosa （85.25%, 52/61）（P 〈 0.05）. The down-regulation or absence of mitotic checkpoint CHFR protein expression was correlated with the sex of GC patients. The absent rate of CHFR protein expression in the female GC patients was significantly higher than that in the male GC ones （64% vs 43.56%, P 〈 0.05）. The absent rates was also significantly different between GC patients of Borrmann Ⅲ ＋ Ⅳand Ⅰ＋ Ⅱtypes （57.14% vs 34.78%, P 〈 0.05）. In the present study, though CHFR protein expression showed no significant difference among various histological types of GC, the absent rate of CHFR protein expression was the highest （71.43%） in signet ring cell carcinoma. The absent expression of CHFR protein was not related to the depth of invasion and lymph node metastasis of GC. In addition, no correlation was found between the expression of CHFR and P53 protein expression in GC （P〉0.05）. CONCLUSION： Down-regulation or absence of mitotic checkpoint CHFR protein expression is frequent events in GC and may take a part in gastric carcinogenesis. Abnormal expression of CHFR may be of more importance in the development of female patients and

关 键 词：CHFR基因 突变型P53 胃癌 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]