胃癌相关基因GCRG213正反向转染和RNA干扰对裸鼠胃癌种植瘤的影响  

Effect of sense or anti-sense GCRG213 fragment and GCRG213 RNA interference on transplanted gastric carcinoma in vivo

作　　者：徐世平[1] 吴本俨[1] 王孟薇[1] 高利利[1] 伍银桥[1] 王卫华[1] 尤纬缔[1] 

机构地区：[1]中国人民解放军总医院南楼消化科,北京市100853

出　　处：《世界华人消化杂志》2007年第14期1639-1642,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.30370635~~

摘　　要：目的:观察胃癌相关基因GCRG213体内正、反向转染和RNA干扰对裸鼠胃癌种植瘤的影响.方法:将对数生长期的胃癌细胞株MKN45接种到裸鼠皮下,成瘤后用制备好的分别含GCRG213正、反义克隆和RNAi片段的腺相关病毒分别在肿瘤局部注射,以空病毒和生理盐水对照,继续喂养2 wk后处死,取肿瘤测量重量,并用半定量RT-PCR检测肿瘤组织GCRG213mRNA表达水平.结果:裸鼠接种MKN45细胞3 wk左右皮下形成肿瘤结节.肿瘤组织重量在正向转染组为5.12±1.02g、反向组1.22±0.46g、RNAi组0.81±0.37g、空病毒组3.13±0.69g、生理盐水组3.45±0.87g;各组GCRG213 mRNA表达水平依次分别为0.406±0.01 3,0.211±0.021,0.087±0.015,0.312±0.050,0.283±0.061.结论:三种腺相关病毒分别有效地介导了GCRG213正、反义克隆和RNAi片段的体内表达,提高或降低了GCRG213 mRNA的表达水平,促进或抑制了胃癌种植瘤的生长.AIM： To investigate the influence of sense or anti-sense GCRG213 fragment and GCRG213 RNA interference （RNAi） on transplanted gastric cancer in vivo. METHODS： Gastric carcinoma cell line MKN45 at logarithmic growth phase was subcutaneously inoculated in 20 athymic mice. After tumor formation, the adeno-associated virus （AAV） containing sense fragment and GCRG213 ment was injected into or anti-sense GCRG213 RNA interference fragtubercles. Meanwhile, empty and normal saline control groups were designed. All the mice were killed 2 weeks after injection. Tumors were weighted and the expression of GCRG213 mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.RESULTS： Three weeks after inoculation of MKN45 cells, small tumor tubercles came into formation. The weights of tumor tissues were 5.12 ± 1.02, 1.22 ± 0.46, 0.81 ± 0.37, 3.13 ± 0.69 and 3.45 ± 0.87 g in sense, anti-sense, RNAi, empty and normal saline group, and the mRNA expression of GCRG213 were 0.406 ± 0.013, 0.211 ± 0.021, 0.087 ± 0.015, 0.312 ± 0.050 and 0.283 ± 0.061 in the above groups, respectively. CONCLUSION： Target genes including sense or antiense GCRG213 and RNAi fragment can be effectively expressed in vivo through AAV vector. Increased or decreased GCRG213 expression can promote or inhibit growth of gastric cancer.

关 键 词：胃癌相关基因GCRG213 腺相关病毒 小干扰RNA 基因转染 胃癌种植瘤 

分 类 号：R735.2[医药卫生—肿瘤]