MMP-9、HPA及CD34在侵袭性垂体腺瘤中表达的研究  

The study on expression matrix metalloproteinase-9,HPA and CD34 in invasive pituitary adenomas

作　　者：李九州[1] 霍钢[1] 郑履平[1] 肖文峰[1] 

机构地区：[1]重庆医科大学附属第一医院神经外科,重庆400016

出　　处：《中国神经精神疾病杂志》2007年第7期404-407,共4页Chinese Journal of Nervous and Mental Diseases

摘　　要：目的检测基质金属蛋白酶-9(MMP-9)、乙酰肝素酶(HPA)及CD34在侵袭性和非侵袭性垂体腺瘤中的表达,探讨其与肿瘤侵袭发生的关系及它们之间的相关性。方法用免疫组化SP法和RT-PCR方法对63例垂体腺瘤患者标本的MMP-9、HPA及CD34的表达进行检测,记数CD34标记的肿瘤内微血管密度(MVD)。结果63例标本中,34例为侵袭性垂体腺瘤,29例为非侵袭性垂体腺瘤,免疫组化显示,侵袭性垂体腺瘤的MMP-9和HPA阳性表达率及强阳性率均高于非侵袭性垂体腺瘤(P均<0.01);侵袭性垂体腺瘤中微血管密度(MVD)也明显高于非侵袭性垂体腺瘤(P(0.01)。RT-PCR法显示,侵袭性垂体腺瘤组MMP-9及HPAmRNA的表达明显高于非侵袭性垂体腺瘤组(P均<0.01);侵袭性腺瘤组中MMP-9与HPAmRNA的表达成正相关(r=0.65,P<0.05),非侵袭性腺瘤组中MMP-9与HPAmRNA的表达无明显相关性(r=-0.32,P>0.05)。结论MMP-9、HPA高表达及高MVD与垂体腺瘤的侵袭性密切相关。MMP-9、MVD和HPA可以作为判别垂体腺瘤侵袭性的有效参考指标。在肿瘤侵袭过程中HPA可能与MMP-9的产生有关。Objective To investigate the expression of MMP-9,HPA and CD34 in patients with invasive pituitary adenomas, and to evaluate the relationship between the expression of MMP-9, HPA, CD34 and the invasion of pituitary adenomas. Methods The expression of MMP-9, HPA and CD34 were examined by immunohistochemical staining and MVD signed by CD34 was counted at the same time,and the expression of MMP-9 and HPA mRNA were examined by RT-PCR assay. Results Thirty-four cases were invasive and 29 were non-invasive in 63 patients with pituitary adenomas. The intense and total expression rate of MMP-9, HPA of pituitary adenomas patients with invasion were significantly higher than those without invasion （P 〈 0. 01 ） ; MVD of pituitary adenomas patients with invasion was significantly higher than that without invasion （P 〈 0. 01 ）. Both mRNA expressions of MMP-9 and HPA were also observed significantly higher in invasive pituitary adenomas than those without invasion （P 〈 0. 01 ）. There was a positive correlation between MMP-9 and HPA mRNA expression in invasive pituitary adenomas（ r = 0. 65 ,P 〈 0.01 ）, and no correlation between them in noninvasive pituitary adenomas （ r = - 0. 32, P 〉 0. 05 ）. Conclusions Higher MMP-9, HPA expression and higher MVD of pituitary adenoms may be useful markers to predict the invasiveness. MMP-9 has closed relationship with the creating of HPA in the invasion of pituitary adenomas.

关 键 词：基质金属蛋白酶-9 乙酰肝素酶 微血管密度 垂体腺瘤 

分 类 号：R736.4[医药卫生—肿瘤]