骨髓基质干细胞对脑胶质瘤大鼠的免疫调节作用  

Immunoregulation effect of bone marrow stromal stem cells on glioma bearing rats

作　　者：李春晖[1] 焦保华[1] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000

出　　处：《中国神经精神疾病杂志》2007年第7期408-411,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：河北省自然科学基金项目(编号:C2004000559)

摘　　要：目的研究骨髓基质干细胞(bone marrowstromal stem cells,BMSCs)在体外及脑荷瘤大鼠体内的免疫调节作用。方法培养BMSCs,以抗原致敏的树突状细胞(dendritic cell,DC)作为刺激细胞,将BMSCs与DC及T淋巴细胞混合培养,或应用Transwell将BMSCs与DC及T淋巴细胞以8μm的膜隔开培养,观察BMSCs对体外混合淋巴细胞反应的调节作用。建立大鼠脑胶质瘤模型,将BMSCs植入荷瘤大鼠脑内,观察对荷瘤大鼠的免疫调节作用。结果BMSCs不能刺激T淋巴细胞的增殖,但明显抑制DC对T淋巴细胞的激活作用;用Transwell将BMSCs与DC及T淋巴细胞以8μm的膜隔开,与BMSCs、DC、及T淋巴细胞混合培养相比,结果无统计学差异(P=0.934);在混合淋巴细胞反应早期加入BMSCs可产生明显的抑制效果,在晚期加入BMSCs则抑制效果明显降低(P<0.01)。流式细胞术检测显示混合淋巴细胞反应的T淋巴细胞凋亡不明显。体内检测显示,BMSCs对荷瘤鼠体内的IFN-γ、IL-2、IL-10水平无明显影响,肿瘤内浸润的淋巴细胞与对照组相比无明显减少(P>0.05)。结论BM-SCs可能通过分泌的细胞因子对T淋巴细胞产生一定的抑制作用。Objective To study the immunoregulation of hone marrow stromal stem cells （BMSCs） in vitro and in vivo. Methods BMSCs and Dendritic cells （DC） were cultured and pulsed with glioma antigen as stimulator. Co-culture BMSCs, DC and T lymphocytes, or by means of Transwell plate, separated BMSCs with DC and T lymphocytes with membrane that had 8μm pores, to observe immunoregulation of BMSCs on mixed lymphocytes reaction. Glioma bearing rats were established followed by BMSCs transplantation for observing in vivo immunoregulation. Results BMSCs could not stimulate T lymphocytes multiplication, but could suppress T lymphocytes activation by DC. Compared co-culture BMSCs, DC and T lymphocytes with separated BMSCs with DC and T lymphocytes with membrane that had 8 μm pores, there were no statistic difference （P=0.934）. During mixed lymphocytes reactions, BMSCs added in early state, compared with BMSCs added in later state, could induce obviously suppress effect （P 〈 0.01 ）. Flow cytometry analysis showed that there were few apoptosis T lymphocytes. In vivo study showed that BMSCs could not influence IL-2, IL-IO and IFN-γ serum concentration in glioma bearing rats. Compared with control group, after BMSCs transplantation, infiltration lymphocytes in glioma were not decreased obviously. Conclusions BMSCs might suppress T lymphocytes multiply in glioma bearing rats by means of secretion of some cytokines.

关 键 词：骨髓基质干细胞 胶质瘤 T淋巴细胞 混合淋巴细胞反应 

分 类 号：R739.4[医药卫生—肿瘤]