伤寒Vi多糖疫苗大规模生产中的工艺改进  

Improved Manufacturing Technique of Vi Polysaccharide Typhoid Vaccine (VPTV)

作　　者：钟泽荣[1] 沈吉友[1] 熊惠玲 金瓯 陈元东 周亮 向时安 龙吉海 黄林 许蓉华 李吟跃 傅慧兰 

机构地区：[1]卫生部成都生物制品研究所

出　　处：《微生物学免疫学进展》1997年第1期20-23,共4页Progress In Microbiology and Immunology

摘　　要：对伤寒Ｖｉ多糖疫苗大规模生产进行了三项工艺改进：１．将液体菌种由静止期接种大罐改为对数生长期接种大罐，大罐培养时间可缩短３－４小时。２．２％（Ｖ／Ｖ）甲醛溶液杀菌时间由５℃６小时改为２０－２５℃２小时，能保证杀菌完全。３．用冷酚精制Ｖｉ多糖由常规法改为乳化法，可减少精制次数及冷酚用量。Three methods of technological improvements in process used for the large scale VPTV as were described follows:1.The fermentation time is shortened by 3-4hrs,using inoculated seed stock suspension in exponential phase instead of in stationary phase;2.Bacteria killed time is shortened to 2hrs with 2% formaline at 20-25℃ instead of 6hrs at 5℃;3。To purify Vi polysaccharide by cold phenol extraction with the emulsified solution instead of by a conuentional method without emulsification can reduce purification times and amounts of phenol consumption and increase the yield of products.

关 键 词：生物制品 伤寒 VI多糖疫苗 生产工艺 

分 类 号：R516.303[医药卫生—内科学] TQ464.1[医药卫生—临床医学]