Vero细胞法检测白喉抗毒素的研究  

Vero Cell Assay for Determination of Diphtheria Antitoxin Titres

作　　者：肖再平[1] 黄宏发[1] 王树巧 谢广中 

机构地区：[1]卫生部上海生物制品研究所

出　　处：《微生物学免疫学进展》1997年第1期43-45,共3页Progress In Microbiology and Immunology

摘　　要：参照Ｍｉｙａｍｕｒａ等报道，建立了微量细胞培养检测白喉抗毒素的方法。以家兔皮肤试验为参照，Ｖｅｒｏ细胞培养法敏感度为９８．１１％，特异度为８４．００％，符合率为９６．２０％，相关系数ｒ＝０．９３。Using the rabbit skin test as the control meliod,the in vitro vero cell assay method and the indirect hemagglutination test (IHA) were used to determine diphtheria antitoxin of 92 freshmen. The antitoxin titres obtained by IHA was compared with those determined by the rabbit skin test,the results confirmed a high correlation between Vero cell assay and rabbit skin test (r=0.93),the sensitivity of Vero cell assay was 98.11%,the accuracy was 96.20%,and the reproducibility was 92.39%. It is concluded that the Vero cell assay method is a highly recommended procedure for diphtheria antitoxin determination.

关 键 词：Vero细胞法 白喉抗毒素 生物制品 鉴定 

分 类 号：R517.103[医药卫生—内科学] R392－33[医药卫生—临床医学]