HPLC法同时测定复方茶碱麻黄碱片中4组分的含量  

Determination of Four components in Compound Theophyiiine Ephedrine Tablets by HPLC

作　　者：汝秋明 李春实 吴萍 

机构地区：[1]吉林省食品药品检验所,长春130033

出　　处：《中国药师》2007年第8期795-796,共2页China Pharmacist

摘　　要：目的:用高效液相色谱法同时测定复方茶碱麻黄碱片中可可碱、盐酸麻黄碱、茶碱和咖啡因的含量。方法:采用Diamonsil C18色谱柱(250mm×4.6 mm,5μm);流动相:甲醇-0.05 mol·L-1磷酸二氢钾溶液-三乙胺(25:75:0.1);流速:0.8 ml·min-1;检测波长:210nm。结果:可可碱进样量在0.06～0.39μg(r=0.999 6)、盐酸麻黄碱进样量在0.03～0.16μg(r= 0.999 6)、茶碱进样量在0.06～0.38μg(r=0.999 6)、咖啡因进样量在0.04～0.23μg(r=0.999 6)范围内呈良好的线性关系。平均回收率分别为100.3%(RSD=0.3%);100.2%(RSD=0.6%);100.2%(RSD=0.6%);100.7%(RSD=0.5%)。结论:本方法简便、准确、重现性好,可用于同时测定复方茶碱麻黄碱片中4组分的含量。Objective： To establish an HPLC method for determination the contents of theophylline, theobromine, ephedrine hydrochloride and caffeine in Compound Theophylline and Ephedrine Tablets. Method： The Diamonsil-C18 column （250 mm × 4.6 mm,5 μm）was used. The methanol-0.05 mol·L^-1 potassium dihydrogen phosphate solution-triethylamine （25：75：0.1）as mobile phase. The flow rate was 0.8 ml·min^-1. The detection wavelength was at 210 nm. Result： The linear ranges of theobromine, ephedrine hydrochloride, theophylline and caffeine were 0.06 -0.39 μg（r =0.999 6） ,0.03 -0.16 μg（r =0.999 6） ,0.06-0.38 μg（r = 0. 999 6） ,0.04 - 0.23 μg（ r = 0.999 6） respectively. The average recoveries（ n = 5 ） were 100.3% , 100.2%, 100.2% and 100.7% with RSD 0. 3% ,0.6% ,0.6% and 0.5% respectively. Conclusion： The method is simple,accurate and sensible. It can be used for quality control of four components in Compound Theophylline and Ephedrine Tablets.

关 键 词：复方茶碱麻黄碱片 可可碱 盐酸麻黄碱 茶碱 咖啡因 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]