生长抑素类似物对大鼠急性胰腺炎PAP的影响  

Influence of somatostatin anologue on pancreatitis-associated protein in rats with acute pancreatitis

作　　者：佟柏峰[1] 周昊昕[1] 邹小明[1] 彭海[1] 周文学[1] 杨维良[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,黑龙江省哈尔滨市150086

出　　处：《世界华人消化杂志》2007年第16期1794-1799,共6页World Chinese Journal of Digestology

基　　金：中华医学会年胰腺外科科研项目基金(2005)~~

摘　　要：目的:观察生长抑素类似物(奥曲肽)对实验性急性胰腺炎(acute pancreatitis,AP)胰腺炎相关蛋白(pancreatitis-associated protein,PAP)的表达及胰腺组织病理变化的影响.方法:Wistar大鼠80只,随机分为阴性对照组、假手术组、手术组、治疗组,每组均在6,12h采集标本.胰腺组织病理切片证实后,对PAP进行半定量RT-PCR及免疫组化检测,其血清做ELISA检测.结果:免疫组化结果显示,手术组PAP表达比假手术组显著增强(x^2=26.11,P<0.01),治疗组比手术组显著降低(x^2=15.65,P<0.05).RT-PCR及ELISA显示,PAP在手术后组织和血清中的表达明显升高,经奥曲肽治疗后明显降低,3组间有显著差异(RT-PCR:F=71.3,P<0.01;ELISA:F=925,P<0.001).免疫组化、RT-PCR及ELISA结果组内比较显示,手术组PAP表达随时间逐渐显著升高,6,12h之间比较有显著差异(x^2=16.92,P<0.05;t= 2.49,P<0.05;t=2.56,P<0.05),而其他组(治疗组ELISA结果除外)6,12h之间无显著差异(P>0.05).手术组病理改变明显.结论:PAP是AP早期表达明显升高的蛋白,对奥曲肽反应敏感,对胰腺炎的诊断和预后判断有一定作用.AIM： To investigate the influence of somatostatin anologue octreotide on the expression of pancreatitis-associated protein （PAP） and pathological changes in pancreas in rats with acute pancreatitis （AP）. Then the level of serum PAP was measured by enzyme-linked immunosorbent assay （ELISA）. METHODS： Eighty Wistar rats were randomized into group A, B, C and D, served as negative control group, sham operation group, AP model group and octreotide-treated group, respectively. The experimental samples were collected at the 6th and 12th hour in all the groups. The expression of PAP in pancreas was detected by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR） and immuno-histochemistry （IHC） after the AP tissues were verified by pathological method. Then the level of serum PAP was measured by enzyme-linked imrnunosorbent assay （ELISA）. RESULTS： IHC showed that the expression of PAP was significantly higher in group C than that in group B （χ^2 = 26.11, P 〈 0.01）, but it was markedly lower in group D than that in group C （2 = 15.65, P 〈 0.05）. RT-PCR and ELISA demonstrated a significant PAP increase in tissue and serum in group C, but a notable decrease in group D, and there were significant differences between group B, C and D （RT-PCR： F = 71.3, P 〈 0.01; ELISA： F = 925, P 〈 0.001）. PAP expression at the 6^th and 12^th hour had obvious difference in group C （IHC：χ^2 = 16.92, P 〈 0.05; RT- PCR： t = 2.49, P 〈 0.05; ELISA： t = 2.56, P 〈 0.05）, but not in the other groups （except the ELISA result）. Dramatic pathological changes were observed in group C. CONCLUSION： PAP highly expressed at the early stage of AP, and it is sensitive to the treatment of octreotide. PAP is also useful for the diagnosis and prognosis of acute partreatitis.

关 键 词：生长抑素类似物 急性胰腺炎 胰腺炎相关 蛋白 大鼠 

分 类 号：R576[医药卫生—消化系统]