细胞色素P4502E1酶与非酒精性脂肪肝的关系  

Association of CYP2E1 enzymes and susceptibility for non-alcoholic liver disease

作　　者：郭艳梅[1] 王沁[2] 祁志[2] 

机构地区：[1]甘肃中医学院临床医疗系,甘肃省兰州市730000 [2]兰州大学第一医院消化科,甘肃省兰州市730000

出　　处：《世界华人消化杂志》2007年第16期1825-1829,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨细胞色素P4502E1(CYP2E1)酶基因多态性与非酒精性脂肪性肝病(NAFLD)的关系及NAFLD的遗传学发病机制.方法:采用聚合酶链反应——限制性片段长度多态性分析法(PCR-RFLP)对40例非酒精性脂肪肝患者,20例酒精性肝病患者,20例非脂肪肝对照者,20例正常对照者分析CYP2E1基因多态性,并进行相关性分析.结果:NAFLD中c2等位基因型与正常对照组(x^2=8.376,P=0.004)和非脂肪肝对照组(x^2= 6.769,P=0.005)相比明显升高,差异有统计学意义(P<0.01),NAFLD中基因型分布与酒精性肝病相比无明显改变,差异无统计学意义(P =0.896).在NAFLD中肝脏病变程度不同,c2等位基因频率不同,在脂肪性肝炎(P=0.04)和肝硬化(P=0.000)中,差异有统计学意义(P<0.05).结论:NAFLD与CYP2E1酶基因多态性有关,同时也为研究NAFLD遗传易感性提供了新的思路.AIM： To investigate correlations between cytochrome P4502E1 （CYP2E1） enzyme gene polymorphisms and non-alcoholic fatty liver disease （NAFLD）. METHODS： A polymerase chain reaction-restric- tion fragment length polymorphism （PCR-RFLP） method was used to determined polymorphisms of CYP2E1 Pst I and Rsa I sites. RESULTS： Genotypes were separated into three： type A （homozygous for c1 gene）, type B （heterozygous for c1/c2 gene）, and type C （ho mozygous for c2 gene）. In 20 healthy controls, cl frequency was 85.0% and c2 15.0%. In 40 patients with NAFLD, cl was 58.8% and c2 41.2%. Comparing the NAFLD group with the healthy controls, cl was decreased and c2 increased, significantly （P = 0.004）. Comparing the NAFLD group with the alcoholic liver disease group, the frequency of the c2 allele gene was not signifi- cantly different （P = 0.896）. In the NAFLD group the frequency of the c2 allele gene increased with the degree of pathogenesis of liver disease （P = 0.000）. CONCLUSION： The genetic polymorphism of CYP2E1 at the Pst I and Rsa I sites is associated with susceptibility for NAFLD.

关 键 词：非酒精性脂肪性肝病 基因多态性 细胞色素P4502E1 

分 类 号：R575.5[医药卫生—消化系统]