凝结芽孢杆菌活菌TBC169株对右旋葡聚糖硫酸钠引发大鼠溃疡性结肠炎的治疗作用  

Therapeutic effects of Bacillus coagulans on the dextran sodium sulfate-induced ulcerative colitis in rats

作　　者：万阜昌[1] 崔云龙 闫述翠 

机构地区：[1]北京天施康医药科技发展有限公司医学部,北京市100037 [2]青岛东海药业有限公司菌种鉴定部,青岛市266400

出　　处：《世界华人消化杂志》2007年第16期1850-1854,共5页World Chinese Journal of Digestology

基　　金：国家科技部863计划项目;NO.2003AA2ZC50~~

摘　　要：目的：研究凝结芽胞杆菌（Bacillus coagulans，BC）对右旋葡聚糖硫酸钠（DSS）引发的大鼠溃疡性结肠炎（UC）的治疗作用．方法：采用DSS引发大鼠UC，分别用10^7和10^6CFU／mLBC、0．02g/mL柳氮磺胺吡啶（SASP）和生理盐水（NS）处理．21d后测定动物体质量、结直肠湿重、肠黏膜溃疡、糜烂点数及面积、髓过氧化物酶活性和肠道菌群培养．结果：治疗21d后，各组中大鼠的体质量增加，以BC10^7CFU／mL增重明显（P〈0．05）；各治疗组中大鼠的结直肠湿重、肠溃疡糜烂点数、面积、MPO活性与模型组比较均有显著性差并（P〈0．05或〈0．01或P〈0．001）：肠道菌群分析，造型后双歧杆菌数明显下降（P〈0．05）．治疗后各组的双歧杆菌数量均明显增加（P〈0．01或P〈0．001），以BC组和SASP组增加最明显（P〈0．01或P〈0．001）．BC在肠道定植．结论：BC对DSS引发的大鼠UC有明显治疗作用．AIM： To investigate the effect of Bacillus coagulans （BC, TBC169 strain） in the treatment of rats with ulcerative colitis induced by dext＇ran sodium sulfate （DSS）. METHODS： DSS was used to induce UC in Sprague Dawley rats, and then the rats were treated with 10^7 CFU/mL, 10^6 CFU/mL BC, 0.02 g/mL sulfasalazine and normal saline （control）, respectively. After 21 days of treatment, the body weight, colorectal wet weight, intestinal mucosal ulcer, erosion spots and area, rnyeloperoxidase （MPO） activity, and intestinal bacterial flora were observed. RESULTS： After treatment, the body weights of rats in all the groups were increased, especially in the group received BC at 10^7 CFU/mL （P 〈 0.05）. Intestinal wet weight, ulcerative number and area, and MPO activity were significantly reduced in all the treatment groups （P 〈 0.05 or P 〈 0.01 or P 〈 0.001） as compared with those in model control group. Analysis on intestinal flora showed that the number of Bifidobacterium was decreased remarkably after modeling, but it was significantly increased in all the treatment groups （P 〈 0.01 or P 〈 0.001）. BC was found colonized in the intestinal tract of BC-treated group CONCLUSION： BC has notable therapeutic effect on DSS-induced UC.

关 键 词：凝结芽胞杆菌 右旋葡聚糖硫酸钠 大鼠 溃疡性结肠炎 肠道菌群 

分 类 号：R574.62[医药卫生—消化系统]