γ-聚谷氨酸发酵培养基的Plackett-Burman法优化  

Optimization of Poly γ-glutamic Acid Fermentation Medium Using Plackett-Burman Experimental Design

作　　者：疏秀林[1] 施庆珊[1] 冯静[1] 欧阳友生[1] 陈仪本[1] 

机构地区：[1]广东省微生物研究所广东省菌种保藏与应用重点实验室,广州510070

出　　处：《生物技术通报》2007年第4期173-177,共5页Biotechnology Bulletin

基　　金：广东省科技攻关项目(2004B10401003);广州市科技计划项目(2005J1-C0051;2006Z3-D0291)

摘　　要：以一株γ-聚谷氨酸高产菌——地衣芽孢杆菌GIM-P10为试验菌株,采用逐因子实验法确定γ-聚谷氨酸合成考察因素的参考范围,再采用Plackett-Burman设计法进行培养基的优化,10个实验因子中筛选到四个显著影响因子:柠檬酸、谷氨酸、K2HPO4和MgSO4·7H2O。另外,综合评价实验结果,表明γ-聚谷氨酸的产量与多糖含量呈负向关系,与细胞干重呈正向关系。利用Plackett-Burman设计法发酵产γ-聚谷氨酸可高达21.27g/L,为基础培养基的2倍以上。A high productive poly γ-glutamic acid strain Bacillus licheniformis GIM-P10 screened by culturing in different mediums.Single-factor designs were used to determine the ranges of value of each related factor which may effect the production of γ-PGA.Then Plackett-Burman factorial design was carried out,to evaluate the effect of different cukure conditions on the production of PGA.Ten variables were examined for their significance on PGA production. Among those variables,citrate,MgSO4·7H2O,L-glutamate and K2HPO4 were found to be the most significant variables that encourage PGA production.The overall trend evaluation of the experimental results revealed a direct proportion between PGA and CDW on one side and the polysaccharide on the other.The optimal medium,based on statistical analysis,showed a production of 21.27g/LPGA,which is more than three times the basal medium.

关 键 词：Γ-聚谷氨酸 地衣芽孢杆菌 培养优化 Plackett-Burman设计法 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]