中国林蛙抗菌肽对胃癌细胞生长抑制作用  

Study on antiproliferative effects of antibiotic peptide from skin of Chinese forest frog on gastric cancer cell lines

作　　者：刘红玉[1] 崔洪斌[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,哈尔滨150081

出　　处：《中国公共卫生》2007年第8期913-914,共2页Chinese Journal of Public Health

基　　金：黑龙江省教育厅科学技术研究项目(11511142)

摘　　要：目的研究中国林蛙抗菌肽对人胃癌细胞SGC-7901生长的抑制作用。方法以人胃癌细胞SGC-7901为体外实验模型,通过细胞形态学观察、四甲基偶氮噻唑蓝(MTT)实验、分裂指数法和集落形成实验,研究抗菌肽对胃癌细胞生长的抑制作用。结果细胞形态学观察,中国林蛙抗菌肽组中,细胞数量减少,细胞膜出现孔洞,浮起的死细胞增加。MTT实验中随着抗菌肽浓度增加,作用时间延长,细胞增殖速度减慢,呈剂量-效应关系和时间-效应关系。分裂指数和集落形成实验中,中国林蛙抗菌肽对胃癌细胞SGC-7901有丝分裂和集落形成均有明显抑制作用(P<0.01)。结论中国林蛙抗菌肽对人胃癌细胞SGC-7901的生长有显著的抑制作用。Objective To study the antiproliferative effects of antibiotic peptide from the skin of Chinese forest frog on gastric cancer cell lines SGC- 7901 in vitro. Methods The gastric cancer cell lines SGC-7901 be used as the model in vitro. The cell morphological observing, MTT, mitotic index experiment and clone formation experiment be adopted to study the inhibition effect of antibiotic peptide. Results In cell morphological observing the cell quantity decreased, holes appeared on the cellular membrane and upfloated dead cell increased in the antibiotic peptide group. In the MTT experiment speed of cell proliferation stepped down as concentration of antibiotic peptide increased and action time extended in dose-and time-dependent. The antibiotic peptide from the skin of Chinese forest frog had significant inhibition effect on cell SGC-7901 mitotic division and clone formation（ P 〈 0.01 ）. Conclusion The antibiotic peptide from the skin of Chinese forest frog has significant inhibition effect on gastric cancer cell lines SGC-7901 proliferation.

关 键 词：抗菌肽 胃癌细胞 抑癌 中国林蛙 

分 类 号：R730.1[医药卫生—肿瘤] R735.2[医药卫生—临床医学]