酶法提取黄芩中黄芩素、汉黄芩素  

Enzymatic Extraction of Baicalein and Wogonin from Radix Scutellariae

作　　者：王宏志[1] 喻春皓[1] 高钧[2] 赵广荣[1] 

机构地区：[1]天津大学化工学院,天津300072 [2]天士力集团有限公司,天津300402

出　　处：《中药材》2007年第7期851-854,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究纤维素酶法提取黄芩中黄芩素、汉黄芩素的最佳工艺条件。方法:采用HPLC法测定黄芩素、汉黄芩素含量,以提取率为指标,分别考察浸提倍数、加酶量、pH、温度、时间对纤维素酶解反应的影响。结果:实验确定了纤维素酶解黄芩药材的最佳工艺条件:浸提倍数20,加酶量20 U/g药材,pH 4.8,温度50℃,时间8 h。结论:与传统水煎提取工艺相比,酶法提取工艺提取使得黄芩中黄芩素提取率提高了5倍、汉黄芩素提取率提高了近4倍。Objective ： The optimum enzymatic extraction of baicalein and wogonin from Radix scutellariae were determined. Methods ： HPLC method was used to determine the content of baiealein and wogoinin from Radix Scutellariae, 5 factors were studied on enzymatic extraction of celluase. Results： The optimum enzymatic extraction of celluase were determined as follows enzyme 20U for lg material, pH 4. 8, buffer to raw material ratio20, temperature 50℃, and reaction time 8 h. Conclusion： Compared with the traditional water extraction, the extraction yield of baicalein and wogonin increase 5 and 4 times.

关 键 词：纤维素酶 提取 黄芩 黄芩素 汉黄芩素 

分 类 号：R282.2[医药卫生—中药学]