急性白血病患者白细胞内谷胱甘肽过氧化物酶与化疗耐药关系的研究  

Study on the Relationship Between Glutathione Peroxidase in Leucocyte and Drug Resistance Chemotherapy in Acute Leukemia

作　　者：贺艳娟[1] 谢兆霞[1] 曹萍[1] 陈蒲荪 胡曼玲[2] 

机构地区：[1]湖南医科大学附属湘雅医院血液病研究室,410008 [2]湖南医科大学卫化教研室

出　　处：《临床血液学杂志》1997年第1期18-20,共3页Journal of Clinical Hematology

基　　金：卫生部科研基金

摘　　要：检测了35例急性白血病患者白细胞内谷胱甘肽过氧化物酶（GSH－PX）活力，同时用抗多药耐药P一糖蛋白单抗JSll－1检测了其中24例患者白细胞耐卜糖蛋白表达。结果显示：化疗前组白细胞内GSH－PX活力（106．28±15．81U）较正常对照组（8．38±7．42）明显增高（P＜0．05）；化疗后组白细胞内GSH－PX活力（125．21±17．75U）较化疗前组明显增高（P＜0．01）；检测24例化疗后患者P－糖蛋白表达16例表达阳性，其中11例疗效差；8例P－糖蛋白表达阴性，其中6例疗效差。P－糖蛋白表达阳性和阴性疗效差者GSH活力均增高。以上结果表明：急性白血病化疗耐药机制，P－糖蛋白高表达是一个较为重要的因素，但不是唯一的，GSH－PX活力增高可能是MDR形成的另一个重要原因。The intracellular activity of glutathione peroxidase (GSH-PX) in 35 patients with acute leukemia (AL) were determined. In the same time,multidrug resistance P-glycoprotein was detected in 24 postchemotherapy patients by immunohistochemical technique using a monoclonal antibody to P-glycoprotein. The results showed that Intracellular activity of GSH-PX (u ) in prechemotherapy patients (106. 28 ± 15. 81 ) were significantly higher than that in normal control (84. 38 ±7. 42) (P < 0. 05 ). Intracellular activity of GSH-PX in postchemotherapy patients (125. 21 ± 17. 75) was significantly higher than that in prechemotherapy patients (P < 0. 005). Among 24 patients treated with combinative chemotherapy, 16 patients had overexpression of P-glycoprotein, 11 in 16 patients were with poor effect;P-glycoprotein was negative in 8 patients,among which 6 patients have repleased multiple times or refractory. But the activity of GSH-PX in P-glycoprotein positive and poor effect negative patients have increased. The above results indicated that the overexpression of Pusglycoprotein is an important mechanisrn of miltidrug resistance in AL.But not the sole,the increasing activity of GSH-PX may be another important factor.

关 键 词：白血病 多药耐药 P-糖蛋白 GSH-PX 

分 类 号：R733.710.5[医药卫生—肿瘤]