环介导恒温扩增技术快速检测大肠杆菌O157特异基因的建立  

The establishment of Loop-mediated isothermal amplification （LAMP） for the detection of specific gene from Bacterium coli

作　　者：刘全英 李延武 高霞 

机构地区：广东省深圳市第二人民医院检验科,518035

出　　处：《中外健康文摘：医药月刊》2007年第7期59-61,共3页

摘　　要：目的 建立环介导的恒温扩增快速检测大肠杆菌O157的方法。方法 采用环介导的恒温扩增反应（LAMP）技术扩增大肠杆菌O157特异性基因,并与传统PCR比较。结果与传统的PCR技术相比,LAMP法更加简便快速、且在等温条件下进行,具有更高的灵敏性;特异性100%。结论 该方法具有灵敏度高,特异性强,操作简便快速,不需要复杂仪器设备、为临床检测大肠杆菌O157提供了一个快速简便的新方法。Objective To establish a method of Loop-mediated isothermal amplification（LAMP） for the detection of specific gene from Bacterium coli in clinic samples. Methods： The specific gene of Bacterium coli 0157 was amplified by LAMP, and compared with PCR. Results： compared with PCR, the LAMP methods is performed under isothermal conditions, its specificity was 100% and the sensitivity was higher than that of PCR. Conclusions： This LAMP-based assay is simple, rapid, sensitive and specific; and no special apparatus is needed which makes it more economical and practical, these results indicate that LAMP can provide a rapid yet simple test for the detection of Bacterium coli 0157.

关 键 词：核酸 环介导等温扩增 大肠杆菌O157 检测 

分 类 号：R446.139[医药卫生—诊断学;医药卫生—临床医学]