淋巴结阴性乳腺癌患者骨髓细胞角蛋白检测及意义  

Detection of Cytokeratin in Bone Marrow in Breast Cancer Patients with Negative Axillary Lymph Nodes and its Significance

作　　者：陈龙舟[1] 王水[1] 赵佳[1] 王风良[1] 

机构地区：[1]南京医科大学第一附属医院

出　　处：《中国肿瘤》2007年第8期639-641,共3页China Cancer

基　　金：淮安市科技局科研资助项目(HAS06052)

摘　　要：[目的]探讨采用免疫细胞化学(ICC)和RT-PCR方法同时检测腋淋巴结阴性乳腺癌(axillary lymph nodes negative breast cancer,ALNNBC)患者骨髓微小转移的意义。[方法]对常规HE染色腋淋巴结阴性的62例乳腺癌患者采用免疫细胞化学(ICC)和RT-PCR方法检测骨髓细胞角蛋白CK19的表达情况。[结果]ICC法检出CK19阳性表达率为14.52%(9/62),而RT-PCR方法检测则CK19阳性表达率为24.20%(15/62),两者差异有统计学意义(χ2=4.1667,P=0.0412)。[结论]常规病理检查腋淋巴结阴性的乳腺癌患者,用ICC法和RT-PCR法对其骨髓血作进一步检测,可以发现微小转移的存在,RT-PCR方法比ICC更敏感。[Purpose] To investigate the significance of concurrent detecting cytokeratin in bone marrow with immunoeytochemistry （ICC） and reverse transeription-polymerase chain reaction （RT-PCR） for breast cancer with micrometastases in bone marrow. [Methods] The expression of cytokeratin 19 （CK19） in bone marrow was detected by ICC and RT-PCR in 62 patients with negative axillary lymph nodes from HE-staining sections. [Results] The expression of CK19 was 14.52%（9/62） by ICC method, and 24.20%（15/62） by RT-PCR. The results were significantly different between the two methods（χ^2=4.1667,P=0.0412）. [Conclusion] Even there is no axillary lympb nodes involvement in routine pathological examination, micrometastases could be detected in bone marrow by ICC and RT-PCH. RT-PCR is superior to ICC.

关 键 词：乳腺肿瘤 微小转移 骨髓 细胞角蛋白 

分 类 号：R737.9[医药卫生—肿瘤]