水蛭桃仁煎剂对血吸虫性肝纤维化小鼠HSC活化影响  

Effects of Decoction of Leech and Peach Kernal on Activation of HSC in Schistosoma Japonicum Infected Hepatic Fibrosis

作　　者：晏丹[1] 舒赛男[2] 

机构地区：[1]武汉科技大学医学院病理教研室,湖北武汉430065 [2]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030

出　　处：《中华中医药学刊》2007年第8期1704-1706,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:观察水蛭桃仁煎剂对肝纤维化小鼠肝星状细胞(HSC)的活化影响,并探讨其对肝纤维化是否具有治疗作用及其作用机制。方法:制备小鼠血吸虫肝纤维化模型,给予水蛭桃仁煎剂灌胃治疗,另设模型对照组和干扰素治疗组。免疫组化检测肝组织的α-平滑肌动蛋白(α-SMA)。结果:经图像分析,中药治疗组和干扰素治疗组α-SMA较模型对照组均显著减少(P<0.01)。结论:抑制HSC的激活可能是水蛭桃仁煎剂抗肝纤维化的机制之一。Objective： To study the effects of Decoction of leech and peach Kernal on activation of HSC in Schistosoma japonicum infected hepatic fibrosis and investigate the possible mechanisms. Methods： The mice fibrosis model was induced by the infection of Sehistosoma Japonicum. The 120 mice were divided randomly into the following groups： the herbal medicine treatment group was treated with 0.5mL Decoction of leech and peach kernel every day; the model contrast group was given an equivalent dose of saline;the interferon treatment group was received of 10MU of IFNα -2b every two days. The expression of α- smooth muscle actin （α- SMA） in liver tissues were assessed by immunohistochemistry. Results - The expression of α - SMA of the herbal medicine treatment group and the interferon treatment group were both more less than the model contrast group （ P 〈0.01 ）. Conclusion： Decreasing the activation of HSC is one of the mechanism of Decoction of leech and peach Kernal against Sehistosoma japonicum infected hepatic fibrosis.

关 键 词：血吸虫肝纤维化 水蛭桃仁煎剂 Α-SMA HSC 

分 类 号：R285.5[医药卫生—中药学]