变链菌细胞外多糖合成与乳牙高龋的关系研究  

Measurement of exopolysaccharides produced by Streptococcus mutans from caries-free and caries-active children

作　　者：支清蕙[1] 林焕彩[1] 廖义东[1] 杨军英[2] 张蓉[1] 

机构地区：[1]广州中山大学光华口腔医学院口腔预防医学教研组,510060 [2]中山大学第一附属医院口腔科

出　　处：《实用口腔医学杂志》2007年第4期492-494,共3页Journal of Practical Stomatology

基　　金：广东省自然科学基因资助项目(编号:04009344)

摘　　要：目的:比较高龋和无龋幼儿变链菌临床分离株在体外利用蔗糖合成水溶性、水不溶性葡聚糖的能力。方法:蒽酮法定量测定从高龋(dmft≥5)和无龋幼儿(dmft=0)牙菌斑分离的变形链球菌临床分离株水溶性和水不溶性葡聚糖产量。结果:高龋组菌株合成水溶性与水不溶性葡聚糖量平均分别为0.0332mg/ml和0.0357mg/ml,高于无龋组菌株的0.0215mg/ml和0.0192mg/ml;高龋幼儿定植的合成水溶性及水不溶性葡聚糖能力强的菌株所占比例也高于无龋幼儿;变链菌临床菌株水不溶性葡聚糖产量平均为0.0301mg/ml,高于水溶性葡聚糖产量(0.0267mg/ml);差异有统计学意义。结论:高龋幼儿的龋活跃性与其口腔中变形链球菌临床菌株合成细胞外多糖能力强有关。Objective： To compare the quantities of water-soluble, water-insoluble glucans synthesized by S. mutans in 3 -4 years old children with high dmft and no caries. Methods： The quantities of water-soluble and water-insoluble glucans synthesized by S. mutans from dental plaques of children with high dmft （dmft≥5） and caries-free （draft = 0） were measured using the anthrone method. Results： S. mutans isolated from the children with high dmft had stronger ability in producing both water-soluble and water-insoluble glucans than those isolated from the cariesfree children （0.0332 mg/ml v.s. 0.0192 mg/ml and 0.0357 mg/ml v. s. 0. 0215 mg/ml respectively）. Children with high dmft harbored more isolates with higher water-soluble or water-insoluble glucans producing ability than caries-free children. Water-insoluble glucans produced by all the clinical isolates is higher than water-soluble glucans （0. 0301 mg/ml v. s. 0.0267 mg/ml） and the difference was statistically significant. Conclusion： There may be a correlation between the extracellular glucans producing ability of S. mutans and the caries susceptibility in deciduous teeth.

关 键 词：龋齿 乳牙 变形链球菌 多糖类 

分 类 号：R788.1[医药卫生—口腔医学]