基因重排检测技术诊断脾脏淋巴瘤  

Diagnosis of splenic lymphoma by gene rearrangements

作　　者：朱少君[1] 李艳红 张伟[1] 王姝妹[1] 巩丽[1] 兰淼[1] 

机构地区：[1]第四军医大学唐都医院病理科,陕西西安710038 [2]第四军医大学唐都医院妇产科,陕西西安710038

出　　处：《第四军医大学学报》2007年第15期1411-1414,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30171052);国家回国人员研究启动基金(Hg98004);陕西省卫生厅科研基金(02D02)

摘　　要：目的：利用B和T淋巴细胞产生的抗原受体分子基因重排检测技术，探讨1例原发性脾脏恶性淋巴瘤（PLS）基因重排检测结果．方法：从组织蜡块中切取组织片5-10张，提取基因组DNA，PCR扩增，聚丙烯酰胺凝胶电泳后银染显示基因重排结果．结果：脾脏淋巴瘤免疫组化证实为B细胞性滤泡性淋巴瘤．从24个不同部位取得的组织经PCR均扩增成功，所有部位组织T细胞受体基因重排阴性，22个部位组织B细胞单克隆性免疫球蛋白基因重排阳性，基因重排阳性率91．7％．结论：应用PCR法检测B淋巴细胞IgH和T淋巴细胞TCR基因重排可作为淋巴细胞来源的良、恶性病变的一种辅助诊断手段，对脾脏淋巴瘤有较高的诊断价值．AIM： To assess the significance of detecting the gene rearrangements of IgH and TCR in the diagnosis of primary mali$1aant lymphoma of the spleens （PLS）. METHODS： Twen- ty-four samples were taken from different places of the PLS. Ge- nomic DNA was extracted, and amplified vht PCR. The products were resolved on denaturing polyacrylamide gels and visualized through silver staining. RESULTS： All samples were successful- ly amplified. Gene rearrangements of IgH and TCR were negative in 10 cases of reactive lymphoid hyperplasia. In all of the 24 sam- ples from PLS, gene rearrangements of TCR У and TCR 13 were not found, and IgH gene rearrangements were found in 22 samples （91.7%）. There was remarkable difference between PLS group and benign group in gene rearrangment （ P 〈 0.05 ）. CONCLU- SION： Detection of the gene rearrangements of IgH and TCR by PCR could be a kind of subsidiary means to diagnose the lympho- proliferative disease. It is very helpful in the diagnosis of splenic lymphoma.

关 键 词：淋巴瘤 基因重排 聚合酶链反应 脾脏 

分 类 号：R733.2[医药卫生—肿瘤]