机构地区:[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室
出 处:《水生生物学报》2007年第4期503-508,共6页Acta Hydrobiologica Sinica
基 金:863计划(2002AA601021);湖北省教育厅重点项目(D200522005);黄石市科技局重点项目资助
摘 要:Zn2+对绿球藻胁迫的实验浓度为0.1、1、10、50、100、200、400mg/L,BG11培养基作对照。实验结果表明,在特定浓度条件下,Zn2+对绿球藻的生长、生理特性以及细胞结构具有显著影响。低浓度Zn2+(0.1—1mg/L)对绿球藻生长基本没有影响;浓度在10—50mg/L时,绿球藻能维持一定的生长速率;但当Zn2+浓度大于100mg/L时,绿球藻的生长受到显著抑制。绿球藻Chla+Chlb以及Chla含量均随培养基中Zn2+浓度的升高而逐渐减少。当Zn2+浓度低于10mg/L时,绿球藻的净光合强度和呼吸强度均随Zn2+浓度的增加而逐渐增加,之后则随浓度的增加而逐渐降低。在设计的浓度下,绿球藻丙二醛含量和过氧化物酶(POD)活性都随培养基中Zn2+浓度的升高而逐渐增强;过氧化氢酶(CAT)和超氧化物歧化酶(SOD)则随Zn2+浓度的增大酶活性先升高后降低。与对照BG11培养基相比,在低浓度,即Zn2+浓度<10mg/L条件下培养的绿球藻,细胞壁无明显增厚,色素变化不大;暴露到高浓度Zn2+的绿球藻,细胞壁明显增厚,蛋白核消失。在≤10mg/L Zn2+浓度下,绿球藻Zn2+的去除率最高为100%;在能维持生长的Zn2+浓度下,去除率均高达80%以上。结果显示,绿球藻是一种耐受Zn2+胁迫的藻类,对锌的去除率也高,可以应用于含锌污水的处理。In the Zn^2+ stress experiments, the concentrations of Zn^2+ were 0.1, 1, 10, 50, 100, 200 and 400 mg/L, BGll culture medium was served as the control. The results showed that Zn^2+ affected markedly on the growth, physiological characteristics and cell structure of Chlorococcum sp. under certain concentration. When the concentration of Zn^2+ was 0.1--1mg/L, the growth of Chlorococcum sp. showed no obvious difference compared with the control. When the concentration of Zn^2+ was 10-- 50mg/L , Chlorococcum sp. could maintain certain growth rate yet. When the concentration of Zn^2+ was higher than 100mg/L, the growth of Chlorococcum sp. was inhibited markedly. The contents of Chl a + Chl b and Chl a decreased gradually with the increase of the concentrations of Zn^2+ in the medium. When the concentration of Zn^2+ was less than 10rag/L, the photosynthesis and respiration of Chlorococcum sp. increased gradually with the increase of Zn^2+ concentrations; and when the concentration of Zn^2+ was higher than 10mg/L, they decreased gradually with the increase of Zn^2+ concentrations. The content of malondiadehyde and activity of peroxidase increased gradually with the increase of Zn^2+ concentrations, and the activities of catalase and superox- ide dismutase increased at first and later decreased with the increase of Zn^2+ concentrations. Compared with the cell cultured in BG11, the cell cultured in low concentrations ( ≤ 10mg/L) showed few changes in pigment and thickness of cell wall. The cell wall of cell cultured in high concentrations of Zn^2+ became thicker, and the pigment decreased and the pyrenoid disappeared. When the concentration of Zn^2+ was ≤10mg/L, the removal rate of Chlorococcum sp. on Zn^2+ reached the maximum and was 100%, and it was above 80% under the concentrations that Chlorococcum sp. could maintain the growth. The results demonstrated that the Chlorococcum sp. could be applied to the treatment of wastewater containing Zn^2+ , because the Chlorococcum sp.
分 类 号:X173[环境科学与工程—环境科学]
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