LC-MS法快速测定大鼠血浆五味子乙素及其药代动力学分析(英文)  

Rapid determination of Schisandrin B by liquid chromatography mass spectrometry and analysis of plasma pharmacokinetics in rat

作　　者：陈玮莹[1] 李慧[2] 罗文鸿[2] 蔡绍先[3] 温博贵[4] 

机构地区：[1]汕头大学医学院生物化学教研室,广东汕头515041 [2]汕头大学医学院中心实验室,广东汕头515041 [3]汕头大学医学院人体解剖教研室,广东汕头515041 [4]汕头大学医学院病理学教研室,广东汕头515041

出　　处：《中国现代医学杂志》2007年第14期1665-1669,1673,共6页China Journal of Modern Medicine

摘　　要：目的建立LC-MS联用测定血浆五味子乙素的方法,观察五味子乙素在Wistar大鼠体内的药代动力学作用。方法提取五味子果实中的有效成分,给予大鼠一次性灌胃,其后在不同时间收集大鼠血液样品,通过LC-MS方法测定大鼠血浆中五味子乙素的浓度,分析大鼠血浆中五味子乙素的浓度-时间数据,应用3p97药代动力学分析软件计算大鼠体内五味子乙素的药代动力学参数。结果本法简单快速,可应用于不同样品中五味子乙素含量的测定。五味子乙素的保留时间为2.96min,血浆平均最大浓度为1.01μg/mL,灌胃后达峰时间为5.65hr,浓度时间曲线下的平均面积为15.98mg/h.L,平均清除率为0.94μg/h。结论本方法可应用于不同样本中五味子乙素的检测和实验动物体内五味子乙素的药代动力学分析。[Objective] To develop a method for determination of Schisandrin B by LC-MS and to observe the pharmacological action of Schisandrin B in Wistar rat.[Methods] The efficacious components was extracted from the fruit of Schisandra chinensis（Turcz.）Baill.The extract was orally administrated to Wistar rat.Blood samples were obtained at different time points after the extract was administrated.The plasma concentration of Schisandrin B was analyzed by LC-MS,and the plasma concentration-time data of Schisandrin B was also analyzed.The parameters of pharmacokinetics of Schisandrin B in the rats were calculated with 3p97 pharmacokinetics program.[Results] The method was simple and rapid for determination of Schisandrin B in different samples.The retention time of the Schisandrin B was 2.96 min.The maximum mean plasma level of Schisandrin B was 1.01 μg/mL which was reached at 5.65 hr after the oral administration.The mean value of area under concentration-time curve（AUC）was 15.98 mg/h·L.The mean clearance（CL）of Schisandrin B in the rat was 0.94 μg/h.[Conclusions] The method can be successfully applied to analysis of Schisandrin B in different samples and to investigating the pharmacokinetics action of Schisandrin B in experimental animals.

关 键 词：大鼠 五味子乙素 LC-MS 药代动力学 

分 类 号：R-332[医药卫生]