紫杉醇节律化疗抗Lewis肺癌血管生成作用的实验研究  

The Antiangiogenic Effect of Metronomic Chemotherapy with Paclitaxel on Murine Lewis Lung Carcinoma

作　　者：韩颖[1] 周芳[1] 蔡政[1] 李醒亚[1] 

机构地区：[1]郑州大学第一附属医院肿瘤科

出　　处：《肿瘤基础与临床》2007年第4期277-279,共3页journal of basic and clinical oncology

基　　金：国家自然科学基金资助项目(编号:30672423)

摘　　要：目的观察紫杉醇节律化疗对C57BL/6小鼠Lewis肺癌(LLC)肿瘤生长及血管生成的影响并探讨其可能的机制。方法建立小鼠LLC模型,随机分组后分别给予紫杉醇节律化疗和生理盐水腹腔注射,隔天测量小鼠体重及肿瘤体积。小鼠处死后称瘤重,应用免疫组化观察小鼠肿瘤组织中微血管和巨噬细胞、NF-κB蛋白的表达。结果节律化疗组小鼠移植瘤的生长速度减慢,对照组皮下瘤重及体积明显大于节律化疗组(P<0.05),节律化疗组微血管和巨噬细胞减少(P<0.05),NF-κB蛋白表达亦减少(P<0.05)。巨噬细胞计数与微血管计数有相关关系。结论紫杉醇节律化疗可明显抑制小鼠LLC的生长及血管生成,其可能机制之一是节律化疗可以下调小鼠肿瘤组织中的NF-κB蛋白表达,从而减少肿瘤新生血管生成。Objective To observe the effect of metronomic chemotherapy with paelitaxel （PTX） on tumor growth and angtogenesis on C57BL/6 mouse Lewis lung eareinoma（LLC） model and its possible mechanisms. Methods LLC models were prepared and randomly divided two groups given according therapy. Mice weight and tumor diameter were measured every other clay. Then tumors were weighed after killed the mice. The average mierovessel dens and maerophage, NF-KB protein expression was determined in tumor tissues of mice by immunohistoehemistry. Results Tumor growth were inhibited by metronomic chemotherapy with PTX. The tumor weight of control group was heavier than metronomic group（P 〈 0.05 ）. The average microvessel dens and macrophage in control group was higher than control group respec- tively（P 〈 0.05） , so as the expression of NF-KB protein（P 〈0.05 ）. Macrophage counts correlates with microvessel dens. Conclusions Metronomic chemotherapy with PTX inhibits tumor growth and angiogenesis in mouse Lewis lung carcinoma model significantly. The potential mechanisms was that metronomic chemotherapy decreased NF-KB protein expression of mouse tumor tissues, so tumor microvessel was inhibited.

关 键 词：节律化疗 紫杉醇 血管生成 NF-ΚB 

分 类 号：R73-36[医药卫生—肿瘤] R734.2[医药卫生—临床医学]