流式细胞术DNA含量分析对胃癌诊断的价值  

The Value of DNA Contents Analysis with Flow Cytometry in the Diagnosis of Gastric Carcinoma

作　　者：赵婷茹[1] 马杰[1] 魏冰[1] 刘明阁[1] 于庆凯[1] 

机构地区：[1]河南省肿瘤医院病理科,河南郑州450008

出　　处：《肿瘤基础与临床》2007年第4期308-310,共3页journal of basic and clinical oncology

摘　　要：目的探讨流式细胞术DNA含量测定对胃癌诊断的价值。方法应用流式细胞仪对60例胃癌胃镜活检标本及20例非癌性病变的活检标本中DNA含量进行测定,并与同期胃镜活检病理结果对比分析。结果胃癌组织中DNA异倍体率为85.0%,而非癌性病变组仅为10.0%,两者之间差异有统计学意义;流式细胞仪DNA含量分析对胃癌诊断的敏感性为85.0%(51/60),特异性为90.0%(18/20)。结论应用流式细胞仪检测胃镜活检标本DNA含量可作为检测胃组织癌变的一种辅助手段。Objective To explore the value of DNA content analysis with flow cytometry（ FCM ） in the diagnosis of gastric carcinoma. Methods To quantitate the DNA of 60 biopsy specimens with gastric carcinoma and 20 biopsy specimens of non-cancer patients, moreover, compared with the synchronization outcome of biopsy through gastroscope. Results The heteroploid rate in gastric cancer was 85.0% ,nevertheless 10.0% was presented in gastric benign lesion, and the sensitivity and specificity to diagnosis of gastric carcinoma with quantitive analysis of DNA through FCM were 85.0% （51/60） and 95.0% （19/20） respectively. Conclusions We can utilize the FCM to quantitate the DNA of biopsy through gastroscope as an assistant method to detect the canceration of gastric tissues.

关 键 词：胃癌 流式细胞仪 DNA含量 

分 类 号：R735.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]