大鼠全脑缺血再灌注后内耳细胞凋亡与NGF、p75的动态表达  

APOPTOSIS OF INNER EAR CELLS AND DYNAMIC EXPRESSIONS OF NGF AND p75 IN RATS AFTER WHOLE CEREBRAL ISCHEMIA-REPERFUSION

作　　者：施宁华[1] 张志坚[1] 王东林[1] 许燕[1] 梁长生[1] 

机构地区：[1]江苏大学分子医学研究所,镇江212001

出　　处：《神经解剖学杂志》2007年第4期423-426,共4页Chinese Journal of Neuroanatomy

基　　金：江苏省教育厅自然科学基金(94170)资助项目

摘　　要：采用大鼠全脑缺血再灌注模型,用原位缺口末端标记法(TUNEL)检测缺血再灌注不同时间内耳的细胞凋亡情况,通过免疫荧光染色观察内耳细胞神经生长因子(NGF)与其低亲和力受体p75的表达和变化,并用透射电镜观察各组大鼠内耳细胞超微结构的改变,探讨NGF与p75在缺血再灌注诱导内耳细胞凋亡过程中的作用。结果显示:再灌注3d时内耳Corti器出现毛细胞凋亡,同时伴有p75与NGF的过表达;随着缺血再灌注时间延长,P75和NGF表达逐渐增强又逐渐减弱。结果提示:p75和NGF参与了脑缺血再灌注诱导的内耳延迟性毛细胞凋亡的过程,p75与NGF的过表达可能起了促进内耳感觉细胞凋亡的作用。The rat model of whole cerebral isehemia-reperfusion was adopted. By terminal deoxynucleotidyl dUTP nick end labeling （TUNEL）, the inner ear cell apoptosis was determinated during different times after isehemia-reperfusion, The expressions and changes of inner ear cells nerve growth factor （NGF） and its low-affinity receptor, p75, were observed by staining. The transmission electron microscopy （ TEM ） was used to observe the ultrastructure changes of the inner ear cells of the rats in different groups, and to evaluate the effects of NGF and p75 in inducement of the inner ear cell apoptosis after ischemia-reperfusion, The results showed that the hair cell apoptosis occurred in the Corti organ of inner ear after 3 d following reperfusion with the over-expression of p75 and NGF simultaneously. With the prolonged time of the ischemia-reperfusion, the expressions of p75 and NGF were becoming stronger and then weaker. These results suggest that p75 and NGF participated in the delayed apoptosis of inner ear hair cells induced by the cerebral ischemia-reperfusion and that the over-expression of p75 and NGF might exert an influence of promoting the apoptosis of sense cells of inner ear.

关 键 词：脑缺血再灌注 内耳 细胞凋亡 NGF P75 

分 类 号：R743[医药卫生—神经病学与精神病学]