胃癌组织和正常胃小凹上皮中抑癌基因E-cadherin hMLH1 APC和MGMT的过甲基化  

Frequent promoter hypermethylation of several tumor suppressor genes in gastric carcinoma and foveolar epithelium

作　　者：蔡建春[1] 刘棣[1] 张海萍[1] 钟山[1] 夏宁邵[2] 

机构地区：[1]福建医科大学附属厦门第一医院肿瘤外科厦门市肿瘤中心,361003 [2]国家传染病诊断试剂与疫苗工程技术研究中心

出　　处：《中华肿瘤杂志》2007年第7期510-513,共4页Chinese Journal of Oncology

基　　金：厦门市科技基金资助项目(3502220052032);厦门市卫生局重点科研基金资助项目(WSK0502)

摘　　要：目的探讨胃癌组织和癌旁正常胃小凹上皮中抑癌基因启动子区的甲基化状况。方法采用甲基化特异性PCR(MSP)方法,检测106例手术切除胃癌石蜡标本的胃癌组织和癌旁正常胃小凹上皮中E-cadherin、hMLH1、APC和MGMT4个抑癌基因启动子区的甲基化状态。结果在胃癌组织中,有1个以上基因甲基化者占72.6%;在正常胃小凹上皮中,有1个以上基因甲基化者占44.3%,二者差异有统计学意义(P<0.01)。甲基化与胃癌组织的Lauren分型、分化程度和pTNM分期均有关(P<0.05),但与Ming's分型无关(P>0.05)。结论E-cadherin、hMLH1、APC和MGMT基因启动子区甲基化普遍存在于胃癌组织和癌旁正常胃小凹上皮中,可能是胃癌发生发展的早期事件。Objective To study the promoter hypermethylation of several tumor suppressor genes in gastric carcinoma （GC） tissue and adjacent normal gastric foveolar epithelium （GFE）. Methods Methylation specific PCR （MSP） was used to examine the promoter methylation of tumor suppressor genes E-cadherin, hMLHI, APC and MGMT in paraffin-embedded gastric cancer tissue and adjacent normal foveolar epithelium in 106 cases. Results The positive rate of genes promoter methylation was 44.3% （47/106 cases） and 72.6% （77/106 cases） at one or more genes tested in the normal GFE and GC tissue, respectively. There was a significant difference in the positive rates of gene promoter methylation between normal GFE and GC tissue （ P = 0. 0001 ）. There was a significant association with Laurén classification, degree of differentiation and pTNM staging in GC （P 〈 0.05 ）, but no significant association with Ming＇s classification （P 〉 0.05 ）. Conclusion Tumor suppressor genes promoter methylation is frequently present in GC and adjacent normal gastric foveolar epithelium, especially in Laurén diffuse type GC, poorly differentiated GC, mucus-secreting （signet ring） cell GC and pTNM stage Ⅲ and Ⅳ GC. Our findings indicate that the gene promoter methylation is a common and early event in GC carcinogensis.

关 键 词：表观遗传学 甲基化 胃肿瘤 抑癌基因 

分 类 号：R735.2[医药卫生—肿瘤]