韧黏素-C的分解与早期肺癌的术后复发转移  

Correlation of tenascin-C degradation fragment with recurrence and/or metastasis in early non-small-cell lung cancer

作　　者：蔡铭[1] 谢宗涛[1] 翁鸢[1] 常庆[1] 常建华[1] 

机构地区：[1]苏州大学附属第四医院无锡市第四人民医院胸外科,无锡214062

出　　处：《中华肿瘤杂志》2007年第7期518-521,共4页Chinese Journal of Oncology

摘　　要：目的探讨细胞外间质韧黏素-C(TN-C)的分解与非小细胞肺癌(NSCLC)术后复发的关系,以及TN-C分解的分子机制。方法收集63例Ⅰ期NSCLC患者的组织标本,以Western blot测定TN-C蛋白的表达,明胶酶谱法测定基质金属蛋白酶(MMPs)的活性。结果63例患者中,12例检测到TN-C的分解片段。有TN-C分解组肺癌组织与正常肺组织MMP-2活性之比为3.5±0.4,明显高于无TN-C分解组(1.5±0.4,P<0.001)。有无TN-C分解与肺癌的复发、转移有关,可检测到小分子TN-C片段者复发、转移率高(P<0.001)。无TN-C分解患者的5年生存率为73.9%,高于有TN-C分解的患者(45.8%,P=0.028)。无TN-C分解患者的4年和10年无复发生存率分别为82.1%和76.6%,高于有TN-C分解的患者(P<0.001)。结论TN-C分解片段是NSCLC术后复发、转移的有效指标,MMP-2可能是肺癌组织中分解TN-C的重要蛋白酶。Objective To study the correlation of tenascin-c（TN-C） degradation with relapse and/ or metastasis in stage- Ⅰ non-small cell lung cancer （NSCLC） in order to search for a potential biomarker for predicting recurrence, and also to investigate the molecular mechanism of TN-C degradation. Methods The fragment of TN-C in 63 surgically treated stage- Ⅰ NSCLC was detected by Western blotting, and the activity of matrix metalloproteinases（MMPs） was also examined by gelatin zymography. Results TN-C degradation fragment was positively detected in 12 of 63 patients, and 9 of these 12 patients （75.0%） were found to develope recurrence during follow-up. The recurrence-free survival at 4 years was 28. 1% in patients with positive TN-C degradation versus 82.1% in those without（P 〈0.001 ）, and which was 76.6% at 10 years in the patients without TN-C degradation. The activity of MMP-2 in the patients with positive TN-C degradation was also found to be significantly higher than that in the patients without （P〈0.001）. Conclusion Tenascin-c degradation fragment may be a reliable biomarker for predicting recurrence and/or metastasis in the early NSCLC, and matrix metalloproteinase-2 may be a responsible proteinase for degradation of tenascin-c.

关 键 词：韧黏素-C 基质金属蛋白酶 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]