鼻咽上皮细胞异型性改变与PCNA及MMP-9表达的相关性研究  

The association of PCNA and MMP-9 expression with carcinogenesis of nasopharyngeal epithelia

作　　者：周小军[1] 王书芹[1] 王士贞[2] 阮岩[2] 邱宝珊[2] 瑞霞[1] 卢标清[1] 

机构地区：[1]广州中医药大学中山附属医院,中山528400 [2]广州中医药大学第一临床医学院

出　　处：《中国中西医结合耳鼻咽喉科杂志》2007年第4期252-255,共4页Chinese Journal of Otorhinolaryngology in Integrative Medicine

基　　金：中国博士后第三十八批基金课题(编号2005038496);广州中医药大学博士后课题(编号2005-12);广东省中医药管理局课题(编号2050061);中山市科技局课题(编号2004A099);中山市医学课题(编号2004019)

摘　　要：目的研究鼻咽上皮细胞癌变过程中不同阶段的增殖细胞核抗原(PCNA)及金属基质蛋白酶9(MMP- 9)表达活性。方法在鼻咽癌高发区,选择慢性鼻咽炎30例、鼻咽上皮细胞不典型增生或化生者42例及鼻咽癌34例作为研究对象,以免疫组化S-P方法检测PCNA及MMP-9表达水平。结果PCNA在鼻咽炎、不典型增生或化生鼻咽上皮细胞、鼻咽原发灶癌组织表达活性分别为10.0%、73.8%、82.4%,而MMP-9在这些组织的表达水平分别为0.0%、38.1%、67.6%,活性表达差异均具有统计学意义(P<0.001)。结论在鼻咽上皮细胞癌变过程中,PCNA及MMP-9依病理分级严重程度而呈现表达增强趋势。Objective To investigate the association of proliferating cell nuclear antigen（PCNA） and matrix metalloproteinases-9（MMP-9） expression with the carcinogenesis process taken place in nasopharyngeal epithelia based on the observation carried out in a group of clinical cases. Methods This study included 30 cases with chronic nasopharyngitis, 42 cases with atypical hyperplasia/metaplasia in nasopharyngeal epithelia and 34 cases with nasopharyngeal carcinoma（NPC）, all chosen from Zhongshan region, where holding a very high incidence of NPC, and being taken a sample from their nasopharynx. Then, the procedures of immunohistochemistry were taken to detect the expression of PCNA and MMP-9 in these nasopharyngeal samples, followed by a comparative analysis on the detected data in this way to explore the differences and implications of PCNA and MMP-9 expressing activities. Results The positive expressing rates of PCNA were 10.0%, 73.8 % and 82.4% in the tissues of nasopharyngitis, nasopharyngeal epithelia with atypical hyperplasia/metaplasia, and NPC focuses, while that of MMP-9 were 0.0%, 38.1%, and 67.6 % in these tissue samples respectively, all these differences with statistical significance （ P 〈 0. 001 ）. Conclusion An increasing activity expression tendency of PCNA and MMP-9 can be concluded with the undergone severity degree in the pathological process of carcinogenesis taken place in nasopharyngeal epithelia.

关 键 词：鼻咽癌前病变 鼻咽癌 增殖细胞核抗原 金属基质蛋白酶 临床研究 

分 类 号：R739.63[医药卫生—肿瘤]