HCG 免疫放射分析法的建立  

Establishment of immunoradiometric assay for human chorionic gonadotropin (HCG) in serum

作　　者：杨建忠[1] 唐国忠[1] 宋爱玲[2] 吴从愿[2] 

机构地区：[1]中国科学院上海原子核研究所 [2]中国医学科学院北京协和医院

出　　处：《核技术》1997年第5期313-316,共4页Nuclear Techniques

摘　　要：采用两种抗ＨＣＧ（人绒毛膜促性腺激素）单克隆抗体，固相试管包被技术建立了ＨＣＧ夹心免疫放射法（ＩＲＭＡ），与人促黄体激素、促卵泡激素和促甲状腺激素均无交叉反应。方法的灵敏度为０．５ＩＵ／Ｌ，批内、批间变异系数分别小于５．４％、６．４％。药盒４℃下存放１个月质量稳定。An immunoradiometric assay (IRMA) for human chorionic gonadotropin (HCG) in serum is established based on two monoclonal antibodies against HCG. There are no cross-reaction with human luteinizing hormone (hLH), follicle stimulating hormone (hFSH) and thyroid stimulating hormone (hTSH). The sensitivity was 0.5U/L, and the recovery for different concentration of HCG was 91.65%￣107.62%. The coefficients of variation for intra and inter-assay were less than 5.4% and 6.4% respectively. 125 I-McAb were stable under the storage of 4℃ for 97 days.

关 键 词：人绒毛膜 促性腺激素 放射免疫测定 HCG 癌 

分 类 号：R730.45[医药卫生—肿瘤]