食品级载体选择标记的研究进展  

Advance in Food-grade Selection Markers

作　　者：黄琴[1] 李卫芬[1] 

机构地区：[1]浙江大学动物科学学院饲料科学研究所教育部动物分子营养学重点实验室

出　　处：《食品与发酵工业》2007年第7期112-118,共7页Food and Fermentation Industries

基　　金：浙江省科研项目资助(No.2006C12086)

摘　　要：乳酸菌、芽孢杆菌等食品级微生物广泛应用于食品、医药、动物养殖等领域并发挥了重要作用。运用基因工程技术对这些微生物进行改良可使其具有更多的功能特性。这些微生物的克隆、表达载体往往含有1个或多个抗生素抗性筛选标记,其抗药性因子的可转移性将危害生物安全。用对人体安全的食品级选择标记替代载体上的抗生素抗性标记可解决这一问题。根据选择的方法,食品级选择标记可分为显性标记和互补标记2大类。文中主要对近十几年来有关食品级选择标记的研究进行综述。Lactic acid bacteria and Bacillus are generally regarded as safe （GRAS） microorganisms and widely used in many fields. We are trying to add additional properties to them by gene engineering. However, the genetically modified （GM） bacteria are not acceptable to use in food, medicine, and animal husbandry due to the presence of antibiotic resistance genes in plasmids. Thus, it is necessary to develop food-grade se lection markers. Food-grade markers can be divided into two classes based on their selection： dominant and complementation markers. The recent developments on these food-grade selection markers were reviewed.

关 键 词：食品级 显性标记 互补标记 

分 类 号：TS20[轻工技术与工程—食品科学]