β-纤维蛋白原-455G/A基因多态性与缺血性脑血管疾病的相关性研究  

Study on the relationship between β-Fibrinogen-455G/A Polymorphism and Ischemic Cerebral Vascular Disease.

作　　者：成俊英[1] 卢丽萍[1] 杜小鹏[1] 汤亚男[1] 丁友法[1] 

机构地区：[1]丽水市人民医院,浙江丽水323000

出　　处：《心脑血管病防治》2007年第4期232-233,238,共3页CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT

摘　　要：目的研究β-纤维蛋白原-455G/A(-βFg-455G/A)基因多态性对血浆纤维蛋白原(fibrinogen,Fg)基因表达的影响及其与缺血性脑血管疾病发病的关系。方法应用聚合酶链反应(PCR)及限制性片段长度多态性分析(RFLP)等方法,检测DNA样品的-βFg-455G/A多态性,采用血浆自动检测系统测定Fg水平。结果病例组A等位基因频率明显高于对照组(P<0.05)。病例组血浆Fg水平明显高于对照组(P<0.01),病例组和对照组中,携带A基因者血浆Fg水平明显增高(P<0.05)。结论-βFg-455G/A等位基因与血浆纤维蛋白原水平相关,A基因携带者缺血性脑血管疾病发病率增高。提示-βFg-455G/A可作为缺血性脑血管疾病的遗传易感标志之一用于易感人群的检测。Objective To study the relationship between β-fibrinogen-455G/A polymorphism, plasma fibrinogen level and ischemie cerebral vacsular disease. Methods Study included 82 patients with ischemie cerebral vascular disase （P group） and 62 healthy people （C group）. For all subjects, the polymorphism of β-fibrinogen-455G/A was analyzed by polymerase chain reaction and restriction fragment length polymorphism （PCR - RELP） technique. Plasma fibrinogen was measured by a computer assistant procedure. Results The frequency of -455A allele in P group was higher than in C group （ P 〈 0.05）. The plasma fibrinogen level was significantly higher in P group than in C group （ P 〈 0.01）. Higher plasma fibrinogen level was shown in all subjects with A - 455 alleles （ P 〈 0.05）. Conclusions The β-fibrinogen-455G/A allele is associated with elevated fibrinogen level and those with A allele are prone to suffer isehernie cerebral vascular disease.

关 键 词：纤维蛋白原 基因多态性 缺血性脑血管病 

分 类 号：R743.3[医药卫生—神经病学与精神病学]