T-DNA插入产生的水稻白化苗突变的遗传分析  

Genetic Analysis of an Albino Mutation by T-DNA Insertion in Oryza sativa L.

作　　者：张泽民[1] 朱海涛[1] 王江[2] 高菊[1] 陈兆贵[1] 刘芳[1] 宛新杉[2] 张景六[2] 张桂权[1] 

机构地区：[1]华南农业大学广东省植物分子育种重点实验室,广东广州510642 [2]中国科学院上海生命科学研究院植物分子遗传国家重点实验室,上海200032

出　　处：《华南农业大学学报》2007年第3期1-5,共5页Journal of South China Agricultural University

基　　金：国家重点基础研究规划基金(G19990116);国家自然科学基金(30571145)

摘　　要：在筛选和鉴定水稻T-DNA(含Basta抗性基因Bar)插入纯合体的过程中,观察到1个白化苗的突变体.对该突变体进行遗传分析表明,分离群体出现绿苗和白化苗2种类型,其分离比率为3∶1,符合1对基因的显性遗传.Basta抗性检测、PCR分子检测及Southern杂交证实,该突变体是由单一T-DNA插入所引起的,白化苗性状与T-DNA共分离.该突变材料可用于插入座位的基因克隆.T-DNA tagging was used to isolate genes in higher plant. In this study, an albino mutant caused by T-DNA insertion in rice was identified. Genetic analysis of the mutant showed that the two types of phenotype, normal and albino in the segregating populations derived from the T-DNA heterozy- gotes, fit the ratio of 3： 1. Test for Basta resistance showed that all of the albino plants were resistant while the normal plants seglegated for resistant and susceptible by the ratio of 2： 1. The results indicated that the albino mutant co-segregated with Basta resistance. The albino mutant caused by T-DNA insertion con- firmed by T-DNA detection using PCR method and Southern blot. This albino mutant was useful for isola- tion of the tagged gene in rice.

关 键 词：水稻 白化苗突变体 T—DNA插入 遗传分析 

分 类 号：Q74[生物学—分子生物学]