大鼠胰腺组织神经胆碱酯酶、弹力纤维和胶原纤维的组合染色  

A combined-staining method for neural cholinesterase and elastic and collagen fibers in rat pancreatic tissues

作　　者：张顺民[1] 郑建明[1] 朱明华[1] 陈伟红[1] 

机构地区：[1]第二军医大学长海医院病理科,上海200433

出　　处：《第二军医大学学报》2007年第8期901-902,共2页Academic Journal of Second Military Medical University

摘　　要：目的:阐明大鼠胰腺组织中的神经胆碱酯酶与弹力纤维和胶厚纤维等分布情况。方法:将大鼠断头处死后取胰腺组织,冰冻连续切片(厚度8μm),选用Karnovsky-Roots和Victoria blue-Ponceau-Picric acid(简称K-R-VB-P-PA法)组合染色法观察其中神经胆碱酯酶、弹力纤维和胶原纤维的分布情况。结果:胰腺组织腺泡间隙、胰岛及其血管的神经胆碱酯酶呈棕红色,弹力纤维呈蓝绿色,胶原纤维呈淡红色,平滑肌纤维呈黄色。结论:K-R-VB-P-PA组合染色法,能够同时观察到胰腺组织中的神经胆碱酯酶、弹力纤维、胶厚纤维和平滑肌纤维的分布情况及其相互伴随关系。Objective：To investigate the distribution of neural cholinesterase and elastic and collagen fibers in rat pancreatic tissues. Methods： Rats were decapitated and the pancreatic tissues were harvested, frozen and made into 8 μm serial sections. A combined-staining method （K-R-VB-P-PA method, a combination of Karnovsky-Roots and Victoria blue-PonceawPicric acid） was used for staining and the distribution of neural cholinesterase and elastic and collagen fibers was observed. Results： The results showed that the neural cholinesterase was brown,the elastic fibers were cyan, the collagen fibers were light red, and the smooth muscle was yellow in the pancreatic acinar, islet of Langerhans and blood vessels. Conclusion： K-R-VB-P-PA combined staining method can demonstrate the distribution of neural cholinesterase,elastic and collagen fibers and smooth muscle fibers in the pancreatic tissues synchronously.

关 键 词：神经组织 胆碱酯酶类 弹性蛋白 胶原 染色与标记 

分 类 号：R361.2[医药卫生—病理学]